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CAS 57360-69-7

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mtt formazan

Description:
MTT formazan is a chemical compound derived from the reduction of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide), a yellow dye commonly used in cell viability assays. The compound appears as a dark purple crystalline solid and is soluble in organic solvents such as dimethyl sulfoxide (DMSO) and ethanol, but is generally insoluble in water. MTT formazan is primarily utilized in biological and biochemical research to assess cell metabolic activity, as viable cells reduce MTT to formazan, resulting in a color change that can be quantitatively measured. The intensity of the purple color correlates with the number of viable cells, making it a valuable tool in cytotoxicity and proliferation studies. Additionally, MTT formazan is stable under standard laboratory conditions, but it is sensitive to light and should be handled with care to prevent degradation. Its use is widespread in various fields, including pharmacology, toxicology, and cancer research, due to its effectiveness in providing insights into cellular health and function.
Formula:C18H17N5S
InChI:InChI=1/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
Synonyms:
  • 4,5-dimethyl-2-{(E)-[(E)-phenyl(phenylhydrazono)methyl]diazenyl}-1,3-thiazole
  • MTT, formazan
  • Methanone, phenyl(2-phenyldiazenyl)-, 2-(4,5-dimethyl-2-thiazolyl)hydrazone
  • 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, Thiazolyl blue formazan
  • Thiazolyl Blue Formazan (MTT Formazan) extrapure AR, 98%
  • N'-anilino-N-[(4,5-dimethyl-1,3-thiazol-2-yl)imino]benzenecarboximidamide
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