CAS 62354-43-2

D-valyl-L-leucyl-L-lysine 4-nitroanilide dihydrochloride

Description:

D-valyl-L-leucyl-L-lysine 4-nitroanilide dihydrochloride is a synthetic peptide derivative commonly used in biochemical research, particularly in studies involving proteolytic enzymes. This compound features a sequence of amino acids, specifically D-valine, L-leucine, and L-lysine, which are linked together, and it includes a 4-nitroanilide moiety that serves as a chromogenic substrate. The dihydrochloride form indicates that the compound is stabilized by the presence of two hydrochloride ions, which can enhance its solubility in aqueous solutions. Characteristically, this substance is often utilized in enzyme assays to monitor protease activity, as the cleavage of the peptide bond releases the chromogenic product, allowing for quantification. Its molecular structure contributes to its specificity and reactivity in biochemical applications. As with many peptide derivatives, it is important to handle this compound with care, following appropriate safety protocols, as it may have specific storage and stability requirements.

Formula: C₂₃H₃₈N₆O₅.2HCl

Synonyms:

• D-Val-Leu-Lys P-Nitroanilide Dihydrochloride

H-D-Val-Leu-Lys-pNA · 2 HCl

CAS: 62354-43-2

Substrate for plasmin and microplasmin. Microplasmin hydrolyzed D-VLK-pNA with a Km of 0.361 +/- 0.017 mM and a kcat of 40.3 +/- 3.3 s⁻¹, whereas native plasmin had a Km of 0.355 +/- 0.002 mM and a kcat of 27.9 +/- 0.3 s⁻¹ (50 mM Tris-HCl, pH 7.4, 0.1 M NaCl, 37 °C).

Formula: C₂₃H₃₈N₆O₅·2HCl

Color and Shape: Light Yellow

Molecular weight: 551.51

Ref: 01-4004781

D-VAL-LEU-LYS P-NITROANILIDE DIHYDROCHLORIDE

CAS: 62354-43-2

Formula: C₂₃H₄₀Cl₂N₆O₅

Purity: 98%

Color and Shape: Solid

Molecular weight: 551.5069

Ref: IN-DA00EBG9

H-D-Val-Leu-Lys-pNA dihydrochloride

CAS: 62354-43-2

Formula: C₂₃H₃₈N₆O₅·2HCl

Color and Shape: White to off-white or light yellow powder

Molecular weight: 551.51

Ref: 7W-GW8955

H-D-Val-Leu-Lys-pNA·2 HCl

CAS: 62354-43-2

D-Val-Leu-Lys-p-nitroanilide is a selective colorimetric substrate for plasmin used to determine plasmin formation from plasminogen in amidolytic activity assays and plasminogen activating assays. Plasmin is a plasma serine protease whose main role is to dissolve fibrin blood clots. After cleavage by plasmin, the protease activity is quantified by the release of p-nitroaniline (pNA) from the substrate.

Formula: C₂₃H₃₈N₆O₅·2HCl

Purity: Min. 95%

Molecular weight: 551.51 g/mol

Ref: 3D-FV110747