Enzymes in Recombinant Proteins

<p>Enterokinase (EK) is a duodenal protease involved in digestion by activating trypsinogen into trypsin, which in turn activates other pancreatic enzymes. EK specifically cleaves after the sequence Asp-Asp-Asp-Asp-Lys (DDDDK↓) and is inactive if a proline follows the site.<br>Recombinant bovine enterokinase (rbEK) shows higher activity than EK from other species and is widely used in biochemical applications. It is a glycosylated single-chain protein of ~200 amino acids, tagged with C-terminal 6×His for Ni²⁺ affinity purification and easy removal after digestion. The purified rbEK has a molecular weight of ~40 kDa and is fully biologically active.</p>

<p>Ribonucleoside-Diphosphate Reductase Subunit M2 (RRM2) belongs to the ribonucleoside diphosphate reductase small chain family.</p>

<p>SUMO protease, also known as Ulp, is a highly specific enzyme that removes small ubiquitin-like modifier (SUMO) from recombinant SUMO fusion proteins. Unlike proteases such as enterokinase or TEV, which recognize linear amino acid sequences, SUMO protease recognizes the native tertiary structure of SUMO, allowing precise cleavage without leaving extra residues at the cleavage site.<br><br>This recombinant SUMO protease is derived from Saccharomyces cerevisiae, expressed in E. coli, and purified to obtain high yields of active enzyme under animal-free conditions. It is well suited for applications in drug and vaccine development, protein manufacturing, and other research uses. A C-terminal 6×His tag is included to enable easy removal of the protease following cleavage via Ni²⁺ affinity purification.</p>

<p>Carboxylesterase 2/CES2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.</p>

<p>Enterokinase (EK) is a serine protease produced in the duodenum and involved in mammalian digestion. It activates trypsinogen to trypsin, thereby indirectly initiating the activation of pancreatic digestive enzymes. EK specifically cleaves after the recognition sequence Asp-Asp-Asp-Asp-Lys, but is inactive if a proline follows the cleavage site.<br>This recombinant bovine enterokinase is the light chain catalytic subunit, comprising a single glycosylated polypeptide chain corresponding to residues Ile801–His1035 of the native protein (Accession # P98072). Expressed in Pichia pastoris under animal-free conditions, the enzyme is highly active and suitable for applications in drug and vaccine development. A C-terminal 6×His tag is included to allow efficient removal by Ni²⁺ affinity chromatography following cleavage reactions.</p>

<p>Striated muscle preferentially expressed protein kinase, also known as aortic preferentially expressed protein 1, APEG-1, SPEG and KIAA1297, is a protein that</p>

<p>Carboxypeptidase B2 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.</p>

<p>Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and play essential</p>

<p>METAP2 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag.</p>

<p>LDHA Protein, Mouse, Recombinant (His) is expressed in E.</p>

<p>PCSK9 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.</p>

<p>B3GALTL is a beta-1,3-glucosyltransferase that transfers glucose to O-linked fucosylglycans on thrombospondin type-1 repeats (TSRs) of several proteins.</p>

<p>HER3/ERBB3 Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag.</p>

<p>dUTPase Protein, Human, Recombinant (His) is expressed in E.</p>

<p>Chymotrypsin-Like Elastase Family Member 3A (CELA3A) is an enzyme that contains one peptidase S1 domain.</p>

<p>CPA2 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.</p>

<p>Arginase 1/ARG1 Protein, Human, Recombinant (His &amp; MYC) is expressed in HEK293 mammalian cells with His and MYC tag.</p>

<p>UDP galactose-4'-epimerase, also known as GALE, enables the body to process a simple sugar called galactose, which is present in small amounts in many foods.</p>

<p>TrkB Protein, Canine, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.</p>

<p>Fumarase is an enzyme that catalyze the reversible hydration/dehydration of fumarate to S-malate and is involved in the tricarboxylic acid or Krebs cycle.</p>