Enzymes in Recombinant Proteins

<p>Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken cEyk)constitute a new receptor tyrosine kinase subfamily.</p>

<p>Tobacco Etch Virus (TEV) protease is a highly site-specific cysteine protease commonly used to remove affinity tags from fusion proteins. It recognizes the sequence Glu-Asn-Leu-Tyr-Phe-Gln↓Gly/Ser (ENLYFQ↓G/S), with cleavage occurring between the Gln and Gly or Ser residue. The most frequently used recognition site is ENLYFQG.<br><br>The optimal cleavage temperature is 30 °C, though conditions such as enzyme amount, incubation time, and temperature may require optimization for different substrates. TEV protease includes an N-terminal 6×His tag, allowing for convenient removal after digestion via Ni²⁺ affinity chromatography.<br><br>The recombinant enzyme consists of 231 amino acids, has a molecular mass of approximately 28.4 kDa, and is fully biologically active following purification.</p>

<p>Cyclophilin C is an enzyme (EC 5.2.1.8) found in both prokaryotes and eukaryotes that interconverts the cis and trans isomers of peptide bonds with the amino</p>

<p>Phosphoglucomutase-2 (PGM2) is a member of PGM family, which catalyzes the inter-conversion of sugar phosphates and participates in anabolic and catabolic</p>

<p>Carbonic anhydrase 5A, mitochondrial, also known as Carbonate dehydratase VA, Carbonic anhydrase VA, CA-VA and CA5A, is a member of thealpha-carbonic</p>

<p>Her4, a member of the EGF receptor family, plays a variety of roles in physiological and pathological states.</p>

<p>TrkC Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.</p>

<p>Mer Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 54 kDa and the accession number is Q12866.</p>

<p>HER3/ERBB3 Protein, Human, Recombinant (His &amp; Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag.</p>

<p>FLT1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag.</p>

<p>FLT1 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.</p>

<p>HER3/ERBB3 Protein, Rhesus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.</p>

<p>ErbB4 Protein, Human, Rhesus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.</p>

<p>HER3/ERBB3 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag.</p>

<p>TrkA Protein, Human, Recombinant (aa 285-413, His) is expressed in E.</p>

<p>HER3/ERBB3 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag.</p>

<p>TrkA Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.</p>

<p>ErbB4 Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag.</p>

<p>TrkA, a tyrosine kinase receptor, is an essential component of the nerve growth factor (NGF) response pathway.</p>

<p>HER3/ERBB3 Protein, Human, Recombinant (aa 20-331, hFc) is expressed in HEK293 mammalian cells with C-Fc tag.</p>