CAS 35013-72-0

Biotin-N-hydroxysuccinimidester

Beschreibung:

Biotin-N-hydroxysuccinimidester, mit der CAS-Nummer 35013-72-0, ist ein Biokonjugationsreagenz, das häufig in biochemischen Anwendungen verwendet wird, insbesondere beim Markieren von Proteinen und anderen Biomolekülen. Diese Verbindung weist eine Biotinstruktur auf, die ein Vitamin ist und eine entscheidende Rolle im Zellstoffwechsel spielt und für ihre starke Affinität zu Avidin und Streptavidin bekannt ist, was sie in verschiedenen Assays und Reinigungstechniken von unschätzbarem Wert macht. Die N-Hydroxysuccinimid (NHS)-Komponente erhöht die Reaktivität des Esters, sodass stabile Amidbindungen mit primären Aminen, die in Proteinen oder Peptiden vorhanden sind, gebildet werden können. Diese Eigenschaft ist besonders nützlich, um biotinilierte Proteine zu erzeugen, die leicht isoliert oder nachgewiesen werden können. Biotin-N-hydroxysuccinimidester ist typischerweise in organischen Lösungsmitteln löslich und zeigt Stabilität unter geeigneten Lagerbedingungen. Seine Anwendungen erstrecken sich auf Bereiche wie Immunologie, Molekularbiologie und Arzneimittelsysteme, wo präzises Markieren und Verfolgen von Biomolekülen für den experimentellen Erfolg entscheidend sind.

Formel: C₁₄H₁₉N₃O₅S

InChl: InChI=1/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1

InChI Key: InChIKey=YMXHPSHLTSZXKH-RVBZMBCESA-N

SMILES: C(CCCC(ON1C(=O)CCC1=O)=O)[C@H]2[C@@]3([C@@](NC(=O)N3)(CS2)[H])[H]

Synonyme:

• (+)-Biotinyl-N-hydroxysuccinimide
• (N-Hydroxysuccinimidyl)biotin
• 1-({5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}oxy)pyrrolidine-2,5-dione
• 1-{[5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoyl]oxy}pyrrolidine-2,5-dione
• 1H-Thieno[3,4-d]imidazole, 2,5-pyrrolidinedione deriv.
• 1H-Thieno[3,4-d]imidazole-4-pentanoic acid, hexahydro-2-oxo-, 2,5-dioxo-1-pyrrolidinyl ester, (3aS,4S,6aR)-
• 2,5-Pyrrolidinedione, 1-[[5-(hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl)-1-oxopentyl]oxy]-, [3aS-(3aα,4β,6aα)]-
• 2,5-Pyrrolidinedione, 1-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]oxy]-
• <span class="text-smallcaps">D</span>-Biotin N-hydroxysuccinimide ester
• Biotin N-hydroxysuccinimide
• Biotin N-hydroxysuccinimidyl ester
• Biotin NHS ester
• Biotin succinimidyl ester
• Biotin-Nhs
• Biotin-OSu
• EZ-Link NHS-Biotin
• Iotin-Nhs
• N-Hydroxysuccinimidobiotin
• N-Succinimidyl <span class="text-smallcaps">D</span>-biotinate
• NHS Biotin
• NSC 345668
• Biotin N-hydroxysuccinimide ester
• D-BIOTIN, SE
• (+)-BIOTINYL-N-SUCCINIMIDYL ESTER
• BIOTIN (LONG ARM) NHS
• NHS-D-BIOTIN
• D-BIOTINYL-OSU
• BIOTIN SE
• BNHS
• D-BIOTIN-N-HYDROXYSUCCINIMIDE ESTER
• D-(+)BIOTIN N-HYDROXYSUCCINIMIDE ESTER
• 5-(2-OXO-HEXAHYDRO-THIENO[3,4-D]IMIDAZOL-6-YL)-PENTANOIC ACID 2,5-DIOXO-PYRROLIDIN-1-YL ESTER
• (+)-BIOTIN N-SUCCINIMIDYL ESTER
• BIOTINYL-N-HYDROXY-SUCCINIMIDE

N-Succinimidyl D-Biotinate

CAS: 35013-72-0

Formel: C₁₄H₁₉N₃O₅S

Reinheit: >97.0%(HPLC)(N)

Farbe und Form: White to Almost white powder to crystal

Molekulargewicht: 341.38

Ref: 3B-S0491

(+)-Biotin N-succinimidyl ester, 98%

CAS: 35013-72-0

In coupling reactions to amino acids, peptides and proteins through primary amines

Formel: C₁₄H₁₉N₃O₅S

Reinheit: 98%

Farbe und Form: Powder or crystals or crystalline powder, White to cream

Molekulargewicht: 341.38

Ref: 02-044771

D-Biotinyl-OSu

CAS: 35013-72-0

The activated biotin derivative Biotin-OSu is used for labeling the amino groups of amino acids, peptides, and proteins. When conducting the biotinylation at pH 6.5, N-terminal amino groups can be selectively tagged in the presence of lysine.

Formel: C₁₄H₁₉N₃O₅S

Reinheit: ≥ 97%

Farbe und Form: White Powder

Molekulargewicht: 341.39

Ref: 01-4014225

2,5-Pyrrolidinedione,1-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]oxy]-

CAS: 35013-72-0

Formel: C₁₄H₁₉N₃O₅S

Reinheit: 98%

Farbe und Form: Solid

Molekulargewicht: 341.3828

Ref: IN-DA00I6XN

Biotin NHS

CAS: 35013-72-0

Biotin NHS (NHS-Biotin) is an amino reactive biotin reagent used to prepare biotinylated surfaces or polypeptides.

Formel: C₁₄H₁₉N₃O₅S

Reinheit: 98.27% - 98.63%

Farbe und Form: Powder

Molekulargewicht: 341.38

Ref: TM-T14579

Biotin NHS

CAS: 35013-72-0

Biotin NHS

Reinheit: ≥98%

Molekulargewicht: 341.38g/mol

Ref: 54-BUP15883

(+)-Biotin N-hydroxysuccinimide ester

CAS: 35013-72-0

Formel: C₁₄H₁₉N₃O₅S

Molekulargewicht: 341.40

Ref: 7W-GV8230

N-Hydroxysuccinimido biotin

CAS: 35013-72-0

N-Hydroxysuccinimido biotin

Reinheit: 98%

Farbe und Form: White To Off White Powder

Molekulargewicht: 341.38g/mol

Ref: 54-BIB101

Biotin NHS

Kontrolliertes Produkt

CAS: 35013-72-0

Stability Moisture Sensitive
Applications Amino reactive biotin reagent for preparing biotinylated surfaces or polypeptides.
References Ngo, T.T., et al.: Applied Biochem. Biotech, 7, 443 (1982), Wojchowski, D.M., et al.: J. Immunol. Methods, 90, 173 (1986);

Formel: C₁₄H₁₉N₃O₅S

Farbe und Form: Neat

Molekulargewicht: 341.38

Ref: TR-S689500

Biotin succinimidyl ester

CAS: 35013-72-0

Biotinylation reagent for labeling nucleic acids or proteins.

Formel: C₁₄H₁₉N₃O₅S

Reinheit: Min. 98 Area-%

Farbe und Form: White Off-White Powder

Molekulargewicht: 341.38 g/mol

Ref: 3D-FB10303

Biotinyl-OSu

CAS: 35013-72-0

Formel: C₁₄H₁₉N₃O₅S

Reinheit: 95%

Farbe und Form: Solid, Off-white powder

Molekulargewicht: 341.38

Ref: 10-F330592

NHS-Biotin

CAS: 35013-72-0

NHS-Biotin is a biotin analog that can be used as an affinity label for the purification of specific proteins. NHS-Biotin is synthesized by reacting monoclonal antibody with lysine residues and a hydroxyl group, which form a covalent bond known as an amide linkage. The NHS-Biotin can then be covalently attached to the protein of interest. This attachment allows the protein to be purified by passing it over a column of immobilized streptavidin. NHS-Biotin has been shown to bind to actin filaments in humans and uptake peptide hormones in the bloodstream. It was also found that human serum contains chemical reactions with NHS-Biotin, forming a disulfide bond between two cysteine residues on the protein.

Formel: C₂₈H₃₉F₃N₆O₉

Reinheit: Min. 95%

Molekulargewicht: 660.64 g/mol

Ref: 3D-DPG-5707

Biotin-OSu

CAS: 35013-72-0

Biotin-OSu is a biotin derivative that is used as a fluorescence probe for the detection of DNA binding activity. It has been shown to bind to DNA with high affinity in an experimental model. Biotin-OSu binds to the major groove of DNA and can be used to detect dna binding activities, such as protein-DNA interactions, by forming a ternary complex with the target protein and a fluorophore. This compound has also been shown to react with mouse monoclonal antibodies and human serum proteins, which are transfer reactions. Biotin-OSu has also been used as a coupling agent in peptide hormones and streptavidin polymerase chain reactions (PCR). The presence of disulfide bonds in this compound give it stability but limits its solubility.

Formel: C₁₄H₁₉N₃O₅S

Reinheit: Min. 95%

Molekulargewicht: 341.39 g/mol

Ref: 3D-BOS-6003-PI