CAS 367-93-1

Isopropyl-β-D-thiogalactopyranosid

Beschreibung:

Isopropyl-β-D-thiogalactopyranosid (IPTG) ist eine synthetische Verbindung, die häufig in der Molekularbiologie als Induktor der Genexpression verwendet wird. Es ist ein Galactosid, spezifisch ein Thiogalactosid, was bedeutet, dass es ein Schwefelatom anstelle des Sauerstoffs im Galactoseanteil enthält. IPTG ist strukturell ähnlich wie Laktose und wird vom lac-Operon-System in Bakterien, insbesondere Escherichia coli, erkannt, wo es an den lac-Repressor bindet und zur Aktivierung von Genen unter der Kontrolle des lac-Promotors führt. Diese Eigenschaft macht IPTG zu einem wertvollen Werkzeug für Forscher in der Proteinexpressionsforschung. Die Verbindung wird typischerweise als weißes kristallines Pulver geliefert und ist in Wasser löslich, was die Vorbereitung von Lösungen für experimentelle Zwecke erleichtert. IPTG ist unter einer Vielzahl von Bedingungen stabil, sollte jedoch an einem kühlen, trockenen Ort aufbewahrt werden, um seine Integrität zu bewahren. Seine Verwendung ist weit verbreitet in der Klonierung und der rekombinanten DNA-Technologie, was die Produktion von Proteinen für Forschungs- und therapeutische Anwendungen erleichtert.

Formel: C₉H₁₈O₅S

InChl: InChI=1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

InChI Key: InChIKey=BPHPUYQFMNQIOC-NXRLNHOXSA-N

SMILES: S(C(C)C)[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O

Synonyme:

• 1-Isopropylthio-β-D-galactopyranoside
• 1-Methylethyl 1-thio-β-<span class="text-smallcaps">D</span>-galactopyranoside
• Galactopyranoside, isopropyl 1-thio-
• Galactopyranoside, isopropyl 1-thio-, β-<span class="text-smallcaps">D</span>-
• Galactopyranoside, isopropyl 1-thio-, β-D-
• Iptg
• Isopropyl 1-thio-β-<span class="text-smallcaps">D</span>-galactopyranoside
• Isopropyl 1-thio-β-<span class="text-smallcaps">D</span>-galactoside
• Isopropyl 1-thio-β-D-galactopyranoside
• Isopropyl 1-thio-β-D-galactoside
• Isopropyl thio-β-<span class="text-smallcaps">D</span>-galactopyranoside
• Isopropyl thio-β-<span class="text-smallcaps">D</span>-galactoside
• Isopropyl thio-β-D-galactopyranoside
• Isopropyl thio-β-D-galactoside
• Isopropyl β-<span class="text-smallcaps">D</span>-thiogalactopyranoside
• Isopropyl β-D-thiogalactopyranoside
• Isopropyl β-thiogalactopyranoside
• Isopropyl β-thiogalactoside
• Isopropyl-β-<span class="text-smallcaps">D</span>-1-thiogalactopyranoside
• Isopropyl-β-D-thiogalaktopyranosid
• Thioisopropyl β-<span class="text-smallcaps">D</span>-galactopyranoside
• Thioisopropyl β-D-galactopyranoside
• isopropil-β-D-tiogalactopiranosida
• β-<span class="text-smallcaps">D</span>-Galactopyranoside, 1-methylethyl 1-thio-
• Isopropylthio-beta-Thiogalactosidase
• Isopropyl-beta-D-thiogalactoside
• β-D-Galactopyranoside, 1-methylethyl 1-thio-
• Isopropyl β-D-1 Thiogalactopyranoside
• Isopropyl β-D-thiogalactoside
• Isopropyl-1-thio-beta-D-galactopyranoside
• HEMIDIOXANE ADDUCT
• Isopropylthio-beta-galactoside
• Isopropyl thiogalactoside
• 1-Methylethyl 1-thio-β-D-galactopyranoside
• Isopropylthio-B-thiogalactopyranoside
• Isopropyl-beta-D-thiogalactopyranoside, Dioxane Free
• 1-methylethyl 1-thio-D-galactopyranoside

Isopropyl-β-D-thiogalactopyranoside, <5ppm dioxane

CAS: 367-93-1

IPTG is a gratuitous beta-D-galactosidase inducer. In Escherichia coli IPTG functions by binding to the lac repressor and altering its conformation. This inactivation prevents the repressor binding to the operator of the lac operon and thus the repression of the beta-galactosidase coding gene lacZ. IPTG is a commonly used reagent in cloning procedures that require induction of beta-galactosidase activity and is used in conjunction with X-Gal or Lapis-β-D-Gal in blue-white color selection of recombinant bacterial colonies.

Formel: C₉H₁₈O₅S

Reinheit: Min. 99.0 Area-%

Molekulargewicht: 238.30 g/mol

Ref: 3D-I-8000

Isopropyl-β-D-thiogalactopyranoside, approx. 15.0% dioxane

CAS: 367-93-1

A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.

Formel: C₉H₁₈O₅S

Molekulargewicht: 238.30 g/mol

Ref: 3D-I-8050

Isopropyl-β-D-thiogalactopyranoside, <5ppm dioxane, plant origin (ex peach gum)

CAS: 367-93-1

A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.

Formel: C₉H₁₈O₅S

Reinheit: Min. 99.0 Area-%

Molekulargewicht: 238.30 g/mol

Ref: 3D-I-8070

Isopropyl-B-D-Thiogalactopyranoside (IPTG) (dioxan-free) for MB, 99%

CAS: 367-93-1

Formel: C₉H₁₈O₅S

Reinheit: min. 99%

Farbe und Form: White, Crystalline powder

Molekulargewicht: 238.31

Ref: SR-67208

Isopropyl-B-D-Thiogalactopyranoside (IPTG) (dioxan free), 99%

CAS: 367-93-1

Formel: C₉H₁₈O₅S

Reinheit: min. 99%

Farbe und Form: White, Crystalline powder

Molekulargewicht: 238.31

Ref: SR-54110

IPTG, 1M aqueous solution

CAS: 367-93-1

A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.

Formel: C₉H₁₈O₅S

Reinheit: Min. 95%

Farbe und Form: Clear Liquid

Molekulargewicht: 238.3 g/mol

Ref: 3D-EI184219