CAS 7240-90-6

X-gal

Beschreibung:

X-gal, oder 5-Brom-4-chloro-3-indolyl-β-D-galactopyranosid, ist eine synthetische Verbindung, die häufig als chromogenes Substrat in der Molekularbiologie verwendet wird. Es ist besonders wertvoll bei der Identifizierung von rekombinanten Bakterien, die das Enzym β-Galactosidase exprimieren. Bei der Hydrolyse durch dieses Enzym produziert X-gal einen blauen Niederschlag, der eine einfache Visualisierung von Kolonien auf Agarplatten ermöglicht. Die Verbindung ist typischerweise ein weißes bis blassgelbes Pulver und ist in Dimethylformamid und Dimethylsulfoxid löslich, aber schlecht in Wasser löslich. X-gal ist unter normalen Laborbedingungen stabil, sollte jedoch an einem kühlen, dunklen Ort gelagert werden, um eine Zersetzung zu verhindern. Seine Verwendung ist weit verbreitet in Klonierungsexperimenten, insbesondere beim Blau-Weiß-Screening, wo es hilft, zwischen rekombinanten und nicht-rekombinanten Klonen zu unterscheiden. Sicherheitsvorkehrungen sollten beim Umgang mit X-gal getroffen werden, da es ein potenzieller Reizstoff sein kann. Insgesamt ist X-gal ein wichtiges Werkzeug in der Gentechnik und Mikrobiologie, da es die Identifizierung genetisch veränderter Organismen erleichtert.

Formel: C₁₄H₁₅BrClNO₆

InChl: InChI=1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI Key: InChIKey=OPIFSICVWOWJMJ-AEOCFKNESA-N

SMILES: O(C=1C=2C(NC1)=CC=C(Br)C2Cl)[C@@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O

Synonyme:

• .beta.-D-Galactopyranoside, 5-bromo-4-chloro-1H-indol-3-yl
• 5-Bromo-4-chloro-1H-indol-3-yl beta-D-galactopyranoside
• 5-Bromo-4-chloro-1H-indol-3-yl β-<span class="text-smallcaps">D</span>-galactopyranoside
• 5-Bromo-4-chloro-3-C-indolyl-β-<span class="text-smallcaps">D</span>-galactopyranose
• 5-Bromo-4-chloro-3-indolyl-.beta.-D-galactoside
• 5-Bromo-4-chloro-3-indolyl-b-D-galactoside
• 5-Bromo-4-chloro-3-indoyl-β-galactopyranoside
• 5-Bromo-4-chloroindol-3-yl β-<span class="text-smallcaps">D</span>-galactopyranoside
• 5-Bromo-4-chloroindol-3-yl β-<span class="text-smallcaps">D</span>-galactoside
• Galactopyranoside, 5-bromo-4-chloroindol-3-yl, β-<span class="text-smallcaps">D</span>-
• Indole, 5-bromo-4-chloro-3-(β-<span class="text-smallcaps">D</span>-galactopyranosyloxy)-
• X-Gal
• Xgal
• β-<span class="text-smallcaps">D</span>-Galactopyranoside, 5-bromo-4-chloro-1H-indol-3-yl
• β-D-Galactopyranoside, 5-bromo-4-chloro-1H-indol-3-yl
• Indole, 5-bromo-4-chloro-3-(β-D-galactopyranosyloxy)-
• Galactopyranoside, 5-bromo-4-chloroindol-3-yl, β-D-
• 5-Bromo-4-chloro-1H-indol-3-yl β-D-galactopyranoside

5-Bromo-4-chloro-3-indolyl β-D-Galactopyranoside [for Biochemical Research]

CAS: 7240-90-6

Formel: C₁₄H₁₅BrClNO₆

Reinheit: >98.0%(HPLC)(N)

Farbe und Form: White to Light gray powder to crystal

Molekulargewicht: 408.63

Ref: 3B-B3201

5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside, 98+%

CAS: 7240-90-6

It is a substrate for beta-galactosidase, which cleaves the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole, which immediately dimerises to give an intensely blue product. It is also used to detect activity of this enzyme in histochemistry and bacteriology. It is employed as a colorles

Formel: C₂₃H₄₁Cu₂N₅O₅

Reinheit: 98+%

Farbe und Form: Powder, White

Molekulargewicht: 594.70

Ref: 02-B21034

5-Bromo-4-chloro-3-(β-D-galactopyranosyloxy)indole

CAS: 7240-90-6

Formel: C₁₄H₁₅BrClNO₆

Reinheit: 98%

Farbe und Form: Solid

Molekulargewicht: 408.6290

Ref: IN-DA003MJ1

5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside

CAS: 7240-90-6

Formel: C₁₄H₁₅BrClNO₆

Reinheit: (HPLC) ≥ 98.0% (anhydrous basis)

Farbe und Form: White to off-white crystalline powder

Molekulargewicht: 408.64

Ref: 7W-GC3237

5-Bromo-4-chloro-3-indolyl-ß-D-Galactopyranoside

CAS: 7240-90-6

M02699 - 5-Bromo-4-chloro-3-indolyl-ß-D-Galactopyranoside

Formel: C₁₄H₁₅BrClNO₆

Reinheit: 98%

Farbe und Form: Solid

Molekulargewicht: 408.63

Ref: 10-M02699

X-Gal

CAS: 7240-90-6

X-Gal

Formel: C₁₄H₁₅BrClNO₆

Reinheit: >98%

Farbe und Form: white powder

Molekulargewicht: 408.63g/mol

Ref: 54-BIMB1001

X-GAL

CAS: 7240-90-6

X-GAL (BCIG) is a widely used substrate of chromogenic β-galactosidase. β-galactosidase cleaves X-GAL and produces an insoluble blue compound.

Formel: C₁₄H₁₅BrClNO₆

Reinheit: 99.23% - 99.65%

Farbe und Form: White Powder

Molekulargewicht: 408.63

Ref: TM-T19075

5-Bromo-4-chloro-3-indolyl b-D-Galactopyranoside

Kontrolliertes Produkt

CAS: 7240-90-6

Stability Moisture, Temperature, Light sensitive
Applications A substrate for β-Galactosidase that is converted by the enzyme into an intense indigo-blue chromophore (615 nm).
References 1. Horwitz, J. et al.: J. Med. Chem. 1964 Jul;7:574-5.Lojda, Z. et al.: Histochemie 1973 Mar 26;34(4):361-9.

Formel: C₁₄H₁₅BrClNO₆

Farbe und Form: Neat

Molekulargewicht: 408.63

Ref: TR-B682350

5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside

CAS: 7240-90-6

X-Gal is a chromogenic substrate for β-galactosidase, yielding a blue precipitate. X-Gal is an indicator for coliforms in culture media, as well as for the detection of coliforms (E. coli) in municipal water supplies and food products. X-Gal can be used in conjunction with the inducer IPTG, which binds and inhibits the lac repressor thus inducing β-galactosidase expression. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
Upon hydrolysis, X-Gal yields a localized, insoluble, blue precipitate, making it exceptionally useful in blotting, immunocytochemical, and ELISA assays. 5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside (X-beta-D-galactoside, X-Gal) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a blue-green colored precipitate.
X-Gal can also be used as a detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the &alpha;-complementation approach. Color formation is due to dimerization and oxidation of 5-bromo-4-chloro-3-hydroxyindole which results from enzymatic cleavage of X-Gal. The reaction proceeds only in the presence of oxygen, i.e. in aerobic cultures. Well detectable color bacterial colonies or liquid cultures is obtained within 16-48 h. In the presence of purified beta-galactosidase, color develops within minutes to a few hours.

Formel: C₁₄H₁₅BrClNO₆

Reinheit: Min. 99.0 Area-%

Molekulargewicht: 408.64 g/mol

Ref: 3D-B-7150

5-Bromo-4-Chloro-3-Indolyl-ß-D-Galactopyranoside (X-Gal) for molecular biology, 98%

CAS: 7240-90-6

Formel: C₁₄H₁₅BrClNO₆

Reinheit: min. 98%

Farbe und Form: White to Off-white, Crystalline powder, Clear, Colourless

Molekulargewicht: 408.61

Ref: SR-10513

5-Bromo-4-Chloro-3-Indolyl-b-D-Galactopyranoside (X-Gal) for tissue culture, 98%

CAS: 7240-90-6

Formel: C₁₄H₁₅BrClNO₆

Reinheit: min. 98%

Farbe und Form: White to Off - white, Crystalline Powder, Clear, Colourless

Molekulargewicht: 408.61

Ref: SR-45904

X-GAL

CAS: 7240-90-6

X-GAL

Reinheit: ≥98%

Molekulargewicht: 408.63g/mol

Ref: 54-BUP17311