CAS 7365-45-9

4-(2-Hydroxyethyl)-1-piperazinethansulfonsäure

Beschreibung:

4-(2-Hydroxyethyl)-1-piperazinethansulfonsäure, allgemein bekannt als HEPES, ist ein zwitterionisches Pufferungsmittel, das in der biologischen und biochemischen Forschung weit verbreitet ist. Es hat einen pKa-Wert von etwa 7,5, was es besonders effektiv macht, um den physiologischen pH-Wert in Zellkulturen und biochemischen Assays aufrechtzuerhalten. HEPES ist in Wasser löslich und zeigt eine gute Stabilität unter physiologischen Bedingungen, was für Experimente, die konsistente pH-Werte erfordern, entscheidend ist. Seine Struktur weist einen Piperazinsring, eine sulfonische Säuregruppe und eine Hydroxyethyl-Seitenkette auf, die zu seiner Pufferkapazität beitragen. HEPES ist nicht toxisch für Zellen, was es für verschiedene Anwendungen geeignet macht, einschließlich der Proteinreinigung und enzymatischer Assays. Darüber hinaus ist es weniger anfällig dafür, biologische Prozesse im Vergleich zu anderen Puffern, wie z.B. Phosphatpuffern, zu stören. Es ist jedoch wichtig zu beachten, dass HEPES UV-Licht absorbieren kann, was bestimmte spektroskopische Analysen beeinträchtigen kann. Insgesamt ist HEPES ein vielseitiger und weit verbreiteter Puffer in Laborumgebungen, insbesondere in den Bereichen Molekularbiologie und Biochemie.

Formel: C₈H₁₈N₂O₄S

InChl: InChI=1/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)/p+1

InChI Key: InChIKey=JKMHFZQWWAIEOD-UHFFFAOYSA-N

SMILES: C(CS(=O)(=O)O)N1CCN(CCO)CC1

Synonyme:

• 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-
• 1-[4-(2-Hydroxyethyl)-1-piperazinyl]ethane-2-sulfonic acid
• 2-[4-(2-Hydroxyethyl)-1-Piperazinyl]Ethanesulfonic Acid
• 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid
• 2-[4-(2-Hydroxyethyl)Piperazin-4-Ium-1-Yl]Ethanesulfonate
• 2-[4-(2-Hydroxyethyl)Piperazinediium-1-Yl]Ethanesulfonate
• 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
• 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
• 2-[4-(2-Hydroxyethyl)piperazinyl]ethanesulfonic acid
• 2-[4-(Hydroxyethyl)-1-piperazinyl]ethane sulfonic acid
• 4-(2-Hydroxyethy1)-1-piperazineethanesulfonic acid
• 4-(2-Hydroxyethyl)-1-Piperazineethanesulfonic Acid
• 4-(2-Hydroxyethyl)-1-Piperazineethanesulfonicacid
• 4-(2-Hydroxyethyl)-1-piperazineethanesulphonic acid
• 4-(2-Hydroxyethyl)Piperazin-1-Ylethanesulphonic Acid
• 4-(2-Hydroxyethyl)Piperazine-1-Ethanesulfonic Acid
• 4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsaure
• 4-(2-Hydroxyethyl)piperazine-1-(2-ethanesulfonic acid)
• Acide 4-(2-Hydroxyethyl)Piperazine-1-Ylethanesulfonique
• Acide 4-(2-hydroxyethyl)piperazin-1-ylethanesulfonique
• Acido 4-(2-Hidroxietil)Piperazin-1-Iletanosulfonico
• Hepes
• Labotest-Bb Lt00138109
• Labotest-Bb Lt00233156
• N-(2-Hydroxyethyl)piperazine-N'-ethanesulfonic acid
• N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)
• N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid), Molecular Biology Grade
• N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid), Tissue Culture Tested
• N-2-Hydroxyethylpiperazine-N-2-ethanesulfonic acid
• N-2-Hydroxyethylpiperazino-N'-2-ethanesulfonic acid
• Nsc 166663
• Piperazine, 1-(Ethylsulfonic Acid)-4- (2-Hydroxyethyl)-
• Tvz 7
• Was 13
• HEPES BUFFER
• N-2-HYDROXYETHYLPIPERAZINE-N'-2-ETHANE SULPHONIC ACID
• HEPES-ACID
• 4-(2-hydroxyethyl)-1-piperazineethanesulfonicaci
• HEPES BUFFERED SALINE
• hepesfreeacid
• GOOD'S BUFFER
• N-(2-Hydroxyethyl)piper
• HEPES BUFFER PH 7.5

2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic Acid [Good's buffer component for biological research]

CAS: 7365-45-9

Formel: C₈H₁₈N₂O₄S

Reinheit: >99.0%(T)

Farbe und Form: White to Almost white powder to crystal

Molekulargewicht: 238.30

Ref: 3B-H0396

HEPES, 1.0M buffer soln., pH 6.5

CAS: 7365-45-9

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Sci

Ref: 02-J61017

HEPES, 1.0M buffer soln., pH 9.0

CAS: 7365-45-9

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Sci

Ref: 02-J60440

HEPES Buffer, 0.2M buffer soln., pH 7.4, low endotoxin

CAS: 7365-45-9

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Sci

Ref: 02-J67485

HEPES, 99%

CAS: 7365-45-9

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research. This Thermo Sci

Formel: C₈H₁₈N₂O₄S

Reinheit: 99%

Farbe und Form: White, Crystals or powder or crystalline powder

Molekulargewicht: 238.30

Ref: 02-A14777

HEPES, 0.5M buffer soln., pH 7.0

CAS: 7365-45-9

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Sci

Ref: 02-J60064

HEPES, 1.0M buffer soln., pH 8.0

CAS: 7365-45-9

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research. This Thermo Sci

Formel: C₈H₁₈N₂O₄S

Farbe und Form: Liquid, Clear colorless

Molekulargewicht: 238.30

Ref: 02-J63578

HEPES, 1M Solution, pH 7.3, Molecular Biology Grade, Ultrapure

CAS: 7365-45-9

Thermo Scientific HEPES Ultrapure

Formel: C₈H₁₈N₂O₄S

Molekulargewicht: 238.30

Ref: 02-J16924

HEPES, 0.5M buffer soln., pH 8.0

CAS: 7365-45-9

HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. It is useful in the pH range 6.8-8.2. HEPES is used as a zwitterionic, all-purpose Good’s buffer for use in biological research. HEPES is widely used in cell culture, larg

Ref: 02-J63002

HEPES, 1.0M buffer soln., pH 7.5

CAS: 7365-45-9

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research. This Thermo Sci

Formel: C₈H₁₈N₂O₄S

Farbe und Form: Liquid, Clear colorless

Molekulargewicht: 238.30

Ref: 02-J60712

HEPES, 0.5M buffer soln., pH 7.5

CAS: 7365-45-9

HEPES is typically used in cell culture at concentration between 5mM to 30 mM. It is used in cell culture media, in biopharma buffer formulations (both upstream and downstream), and in diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins

Ref: 02-J61275

HEPES-buffered saline, pH 7.0 (5X)

CAS: 7365-45-9

HEPES is widely used in cell culture. HEPES is used to neutralize the complex proteins in growth medium that may inactivate trypsin during trypsinization. N-2-hydroxyethylpiperazine-N-2?-ethanesulfonic acid (Hepes) has been tested as an alternative to tris-(hydroxymethyl)aminoethane (Tris) for hydro

Formel: C₈H₁₈N₂O₄S

Farbe und Form: Clear, colorless liquid

Molekulargewicht: 238.30

Ref: 02-J60753

HEPES, 0.5M buffer soln., pH 8.5

CAS: 7365-45-9

HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. It is useful in the pH range 6.8-8.2. HEPES is used as a zwitterionic, all-purpose Good’s buffer for use in biological research. HEPES is widely used in cell culture, larg

Ref: 02-J63218

HEPES, 0.5M buffer soln., pH 7.6

CAS: 7365-45-9

HEPES is typically used in cell culture at concentration between 5mM to 30 mM. It is used in cell culture media, in biopharma buffer formulations (both upstream and downstream), and in diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins

Ref: 02-J61047

HEPES

CAS: 7365-45-9

Formel: C₈H₁₈N₂O₄S

Reinheit: 97.0%

Farbe und Form: Solid, Powder or Crystalline Powder or Solid

Molekulargewicht: 238.3

Ref: 10-F047861

HEPES, ≥99% (Dry Basis), Ultrapure

CAS: 7365-45-9

Thermo Scientific HEPES Ultrapure

Formel: C₈H₁₈N₂O₄S

Reinheit: 99%

Farbe und Form: White crystalline powder

Molekulargewicht: 238.30

Ref: 02-J16926

HEPES-buffered saline, pH 7.0 (2X for transfection)

CAS: 7365-45-9

HEPES (2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid)-buffered saline, 7365-45-9, is a buffer used to maintain the pH of cell culture. Learn more.

Formel: C₈H₁₈N₂O₄S

Farbe und Form: Clear colorless, Liquid

Molekulargewicht: 238.30

Ref: 02-J62623

HEPES-buffered saline, pH 6.5 (5X)

CAS: 7365-45-9

HEPES is typically used in cell culture at concentration between 5mM to 30 mM. It is used in cell culture media, in biopharma buffer formulations (both upstream and downstream), and in diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins

Formel: C₈H₁₈N₂O₄S

Molekulargewicht: 238.30

Ref: 02-J61239

HEPES, 1.0M buffer soln., pH 7.0

CAS: 7365-45-9

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research. This Thermo Sci

Formel: C₈H₁₈N₂O₄S

Farbe und Form: Clear colorless, Liquid

Molekulargewicht: 238.30

Ref: 02-J62688

HEPES, 1.0M buffer soln., pH 8.5

CAS: 7365-45-9

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Sci

Ref: 02-J61360