6-Chloro-3-indoxyl-β-D-galactopyranoside

CAS: 138182-21-5

Ref. 3D-C-5000

Produktinformation

• Name: 6-Chloro-3-indoxyl-β-D-galactopyranoside
• Synonyme: Salmon-beta-D-galactosideEC05439
• Marke: Biosynth
• Beschreibung: 6-Chloro-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase that yields a salmon or rose-coloured precipitate and hence is also known as Salmon-Gal. It can be used in conjunction with isopropyl-beta-D-thiogalactopyranoside (IPTG) as an alternative to the chromogenic substrate 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside (X-Gal) for detection of beta-galactosidase activity in bacterial colonies in colorimetric assays. Where beta-galactosidase expressed from a reporter gene in molecular biology experiments, recombinant colonies on growth plates are salmon-coloured while non-recombinants appear as white. Use of nitroblue tetrazolium salt (NBT) with Salmon-Gal may provide faster results, yielding a clear, purple precipitate, than for Salmon-Gal alone. In conjunction with 5-bromo-4-chloro-3-indolyl-beta-D-glucoside (X-Glc), Salmon-Gal is useful for simultaneous detection of GUS and Lac activities in the same medium.

Chemische Eigenschaften

• Molekulargewicht: 329.74 g/mol
• Formel: C₁₄H₁₆ClNO₆
• Reinheit: Min. 98.0 Area-%