DNA damage-binding protein 2 (DDB2)-[Cys(AF647)]-amide

Ref. 3D-CRB1100404

Produktinformation

• Name: DNA damage-binding protein 2 (DDB2)-[Cys(AF647)]-amide
• Synonyme: H-SIVRTLHQHKLGRASWPSVQQGLQQS(C/AF647)-NH2SIVRTLHQHKLGRAS-W-PSVQQGLQQS-[Cys(AF647)]-amide,H-Ser-Ile-Val-Arg-Thr-Leu-His-Gln-His-Lys- Leu-Gly-Arg-Ala-Ser-Trp-Pro-Ser-Val-Gln-Gl n-Gly-Leu-Gln-Gln-Ser-[Cys(AF647)]-NH2

Beschreibung

DNA damage-binding protein 2 is able to recognise and bind to UV-induced DNA lesions and facilitates efficient recognition by XPC. DDB2 and XPC then initiate global genome nucleotide excision repair (GG-NER) to protect DNA from mutagenesis associated with premature aging and skin cancer. Timely DDB2 dissociation is required for DNA damage handover to XPC and swift progression of the multi-step repair reaction. Damage handover from DDB2 to XPC coincides with the arrival of the TFIIH complex, which further promotes DDB2 dissociation and formation of a stable XPC-TFIIH damage verification complex. DDB2 binds directly to and flips out UV-induced damaged bases to create a more suitable substrate for XPC.The UV-DDB complex is part of a larger E3 ubiquitin-ligase complex (CRL4DDB2), also containing CUL4A, RBX1, and the COP9 signalosome. AF647 dye is a commonly used bright, far-red-fluorescent dye which is pH-insensitive over a wide molar range.

• Marke: Biosynth

Chemische Eigenschaften

• Molekulargewicht: 4,023.6 g/mol