5-Carboxytetramethylrhodamine succinimidyl ester

CAS: 150810-68-7

Ref. 3D-FC45651

Produktinformation

• Name: 5-Carboxytetramethylrhodamine succinimidyl ester
• Synonyme: 5-TAMRA SE, Xanthylium, 9-2-carboxy-4-(2,5-dioxo-1-pyrrolidinyl)oxycarbonylphenyl-3,6-bis(dimethylamino)-, inner salt, 5-Carboxytetramethylrhodamine, 5-Tamra,Se, 2-[6-(dimethylamino)-3-(dimethyliminio)-3H-xanthen-9-yl]-5-{[(2,5-dioxopyrrolidin-1-yl)oxy]carbonyl}benzoate

Beschreibung

5-Carboxytetramethylrhodamine succinimidyl ester is a widely employed fluorescent dye (orange/red) that is suitable for different uses. In PCR applications, it has been used as both a reporter at the 5’ or as a quencher at the 3’ end. TAMRA-labelled oligonucleotides have been used to study interactions with DNA or DNA/protein interactions. It is an excellent fluorescent acceptor for FRET applications with a suitable dye pair. It is used as a protein/antibody labelling agent for structural studies, immunochemistry and flow cytometry. Bioconjugation is generally performed through coupling of the carboxylic acid to a primary amine at the N-terminus of a protein, on lysine side chains or amino-modified oligonucleotide. Carboxylic acids that are activated with succinimidyl esters react with primary amines.

• Marke: Biosynth

Chemische Eigenschaften

• Molekulargewicht: 527.52 g/mol
• Formel: C₂₉H₂₅N₃O₇
• Reinheit: Min. 95 Area-%
• Farbe/Form: Red Powder