5-Fluorescein isothiocyanate isomer I - 95%

CAS: 3326-32-7

Ref. 3D-FF175095

Produktinformation

• Name: 5-Fluorescein isothiocyanate isomer I - 95%
• Synonyme: 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-5-isothiocyanatobenzoic acid, 3′,6′-Dihydroxy-5-isothiocyanatospiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, 3′,6′-Dihydroxy-6-isothiocyanatospiro[2-benzofuran-3,9′-xanthene]-1-one, 4-Isothiocyanatofluorescein, 5-Fitc, 5-Isothiocyanato fluorescein, 5-Isothiocyanatofluorescein, Fitc 'Isomer I', Fitc Isomer, Fitc isomer 1, Fitc-Celite(R), Fluorescein 5-isothiocyanate, Fluorescein Isomer I Isothiocyanate, Fluorescein Isothiocyante, Fluorescein isothiocyanate, isomer 1, Fluorescein, 5-isothiocyanato-, Fluoroscein-5-isothiocyanate, Spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, 3′,6′-dihydroxy-5-isothiocyanato-, Fluorescein isothiocyanate isomer I

Beschreibung

5-Fluorescein isothiocyanate isomer I (5FITC) is a fluorescent probe that has been used to study enzyme activities in human serum. 5FITC binds to the active site of many enzymes, including dopamine β-hydroxylase, and can be detected by measuring the fluorescence intensity. The optimum concentration for this enzyme form is 10 nM for dopamine β-hydroxylase and 20 nM for brain functions. Mouse monoclonal antibodies against 5FITC have also been developed to detect the presence of toll-like receptor 4 (TLR4) on macrophages in vivo. 5FITC has also been used as a fluorescence probe for human immunoglobulin G (IgG) and erythrocytes in blood samples. Colloidal gold conjugates with 5FITC have been developed to measure the disulfide bonds in proteins such as human serum albumin.

• Marke: Biosynth

Chemische Eigenschaften

• Molekulargewicht: 389.38 g/mol
• Formel: C₂₁H₁₁NO₅S
• Reinheit: Min. 95%
• InChl: InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI Key: InChIKey=MHMNJMPURVTYEJ-UHFFFAOYSA-N
• SMILES: O=C1OC2(c3ccc(O)cc3Oc3cc(O)ccc32)c2ccc(N=C=S)cc21