Produktinformation
- Galactopyranosyl fluoride, β-D-
- β-D-Galactopyranosyl fluoride
The enzyme b-D-galactopyranosyl fluoride is a glycosylase that catalyzes the removal of a D-galactose from the 3 position of a β-D-galactopyranosylsucrose. This enzyme is found in bacteria, such as E. coli and B. subtilis. The rate of the reaction is enhanced by fluoride ions, which are required for catalysis. The second order rate constants for the reaction have been determined using wild-type and mutant enzymes expressed in E. coli cells. The values obtained were 0.0025 ± 0.0005 and 0.002 ± 0.001 s−1, respectively, corresponding to an activation energy of 12 ± 4 kJ mol−1 (kcal mol−1).