5-(Iodoacetamido)fluorescein

CAS: 63368-54-7

Ref. 3D-FI11400

Produktinformation

• Name: 5-(Iodoacetamido)fluorescein
• Synonyme: 5-Iodoacetamidofluorescein (5-IAF), N-(3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthen]-5-yl)-2-iodoacetamide, 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-4-[(iodoacetyl)amino]benzoic acid

Beschreibung

5-(Iodoacetamido)fluorescein is a fluorescent probe that can be used as a substrate for the detection of proteolytic enzymes. It binds to the disulfide bond of serine protease and reacts with iodoacetic acid. This reaction leads to a biphasic response, with an initial fluorescence increase followed by a decrease in fluorescence intensity. The initial fluorescence increase is due to the formation of a stable 5-iodoacetylamino-2-aminopyridine intermediate, which is converted into 5-iodoacetamido-2-aminopyridine upon cleavage of the disulfide bond. Fluorescence emission from 5-(Iodoacetamido)fluorescein increases when it binds to tryptophan residues on actin filaments, which are found in large numbers in human liver cells. 5-(Iodoacetamido)fluorescein also binds to herpes simplex virus and

• Marke: Biosynth

Chemische Eigenschaften

• Molekulargewicht: 515.25 g/mol
• Formel: C₂₂H₁₄INO₆
• Reinheit: Min. 95%
• Farbe/Form: Red Powder