IsoTag™ AAV - Residual Reagent ELISA

Ref. 5M-F1035

Produktinformation

• Name: IsoTag™ AAV - Residual Reagent ELISA

Beschreibung

The IsoTag™ AAV Residual Reagent assay is a two-site immuno-enzymatic assay intended to quantify IsoTag™ AAV Residual Reagent. The F1035 kits incorporate a well-qualified sample treatment method to dissociate the IsoTag™ AAV Residual Reagent from the AAV virus. Samples potentially containing residual IsoTag™ AAV Reagent are mixed with sample denaturing buffer to dissociate the biopolymer from the AAV virus. The samples are then reacted simultaneously with a horseradish peroxidase (HRP) enzyme labeled anti-IsoTag™ AAV Reagent antibody (goat polyclonal) in microtiter strips coated with an affinity purified capture antibody (goat polyclonal). After a wash step to remove any unbound reagents, the strips are then reacted with tetramethylbenzidine (TMB) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of IsoTag™ AAV Residual Reagent present in the sample. This immunoassay method provides sensitivity to the IsoTag™ AAV Residual Reagent to less than 1ng/mL. This kit can be used as a tool to aid in optimal purification process development and in routine quality control of in-process manufacturing as well as final product.

• Marke: Cygnus