EasyStep Human ANXA1(Annexin A1) ELISA Kit

Ref. EK-ELK023ES

Produktinformation

• Name: EasyStep Human ANXA1(Annexin A1) ELISA Kit
• Synonyme: ANX-A1; ANX1; LPC1; Lipocortin I; Chromobindin-9; Calpactin II; Phospholipase A2 inhibitory protein

Beschreibung

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ANXA1, and the Human ANXA1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human ANXA1. After TMB substrate solution is added, only those wells that contain Human ANXA1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ANXA1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marke: ELK Biotechnology