Mouse AGER(Advanced Glycosylation End Product Specific Receptor) ELISA Kit

Ref. EK-ELK3005

Produktinformation

• Name: Mouse AGER(Advanced Glycosylation End Product Specific Receptor) ELISA Kit
• Synonyme: RAGE; Receptor For Advanced Glycation Endproducts

Beschreibung

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse AGER. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AGER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse AGER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AGER in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marke: ELK Biotechnology