Human CGb(Chorionic Gonadotropin β Polypeptide) ELISA Kit
Ref. EK-ELK5460
48T | Ausgelaufen | ||
96T | Ausgelaufen |
Produktinformation
- CG-B; CGB3; hCGB; HCG; CGb5; CGb7; CGb8; Choriogonadotropin subunit beta
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CGb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CGb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CGb in the samples is then determined by comparing the OD of the samples to the standard curve.