Dog ET-1(Endothelin1) ELISA Kit

Ref. EK-ELK5700

Produktinformation

• Name: Dog ET-1(Endothelin1) ELISA Kit
• Synonyme: ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1

Beschreibung

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marke: ELK Biotechnology