Pig MMP1(Matrix Metalloproteinase 1) ELISA Kit

Ref. EK-ELK7380

Produktinformation

• Name: Pig MMP1(Matrix Metalloproteinase 1) ELISA Kit
• Synonyme: CLGN; CLG1; Collagenase; Interstitial Collagenase; Vertebrate Collagenase; Fibroblast Collagenase

Beschreibung

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig MMP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig MMP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig MMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig MMP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marke: ELK Biotechnology