Cattle bHB(β-Hydroxybutyric Acid) ELISA Kit

Ref. EK-ELK8618

Produktinformation

• Name: Cattle bHB(β-Hydroxybutyric Acid) ELISA Kit
• Synonyme: OHB; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid

Beschreibung

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle bHB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle bHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle bHB in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marke: ELK Biotechnology