Dog GAL3(Galectin 3) ELISA Kit

Ref. EK-ELK9530

Produktinformation

• Name: Dog GAL3(Galectin 3) ELISA Kit
• Synonyme: LGALS3; CBP35; GALBP; GALIG; MAC2; 35 kDa lectin; Carbohyd犬e-binding protein 35; Galactose-specific lectin 3; Galactoside-binding protein; Laminin-binding protein; Lectin L-29

Beschreibung

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog GAL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog GAL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog GAL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog GAL3 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marke: ELK Biotechnology