10X Tris-Glycine-SDS Buffer for molecular biology

Ref. SR-57806

Produktinformation

• Name: 10X Tris-Glycine-SDS Buffer for molecular biology
• Marke: Sisco Research Laboratories
• Beschreibung: SDS-PAGE is a method for separating proteins according to their movement in an electrical current and their molecular mass, which relates to the length of their polypeptide chains. A widely recognized system for this technique is the Laemmli system, introduced in 1970. It employs Tris-Glycine gels made up of a stacking gel and a resolving gel, utilizing varying acrylamide concentrations to achieve separation based on molecular weight. This traditional system incorporates a discontinuous buffer setup, with different pH levels and ionic strengths for the buffers: the running buffer (10X Tris-Glycine-SDS Gel Running Buffer) is set at pH 8.3, while the stacking gel operates at pH 6.8, and the resolving gel is at pH 8.8.

Chemische Eigenschaften

• Farbe/Form: Clear, Colourless, Liquid