20X SSPE Buffer pH-7.4 suitable for molecular biology

Ref. SR-78305

Produktinformation

• Name: 20X SSPE Buffer pH-7.4 suitable for molecular biology
• Marke: Sisco Research Laboratories
• Beschreibung: The 20X SSC Buffer, with a pH between 6.9 and 7.1, is essential for in molecular biology, particularly for Southern and Northern hybridization techniques. This solution is sterilized through a 0.22 µm filter, making it suitable for direct use or for dilution based on experimental requirements. In parallel, 20X SSPE is available as a concentrated buffer intended for nucleic acid hybridizations and blot transfer processes. A significant feature of SSPE is its inclusion of EDTA, which chelates divalent metal ions like Mg²+. This property effectively inhibits DNase activity, helping to preserve the concentrations of probe and target DNA during Southern hybridization, thereby enhancing the accuracy of the results.

Chemische Eigenschaften

• Farbe/Form: Clear, Colourless, Liquid