CAS 1158-17-4

o-Nitrofenil β-D-tiogalactósido

Descripción:

o-Nitrofenil β-D-tiogalactósido (comúnmente abreviado como ONPG) es un compuesto sintético ampliamente utilizado como sustrato en ensayos bioquímicos, particularmente en el estudio de la actividad enzimática, como la β-galactosidasa. Es un sólido cristalino incoloro que es soluble en agua y disolventes orgánicos. ONPG se caracteriza por su capacidad para liberar o-nitrofenol tras la hidrólisis, que puede medirse cuantitativamente debido a su color amarillo, lo que lo convierte en un indicador útil en ensayos enzimáticos. El compuesto presenta un enlace tiogalactósido, que es susceptible a la escisión enzimática, lo que permite a los investigadores estudiar la cinética y los mecanismos de las enzimas glicosidasas. Su estructura molecular incluye un grupo nitrofenilo, que contribuye a sus propiedades espectroscópicas. ONPG se utiliza a menudo en microbiología para la detección de la fermentación de lactosa y en biología molecular para la evaluación de la expresión génica. Se deben observar precauciones de seguridad al manipular ONPG, al igual que con muchas sustancias químicas, debido a la posible toxicidad y el impacto ambiental.

Fórmula: C₁₂H₁₅NO₇S

InChI: InChI=1/C12H15NO7S/c14-5-7-9(15)10(16)11(17)12(20-7)21-8-4-2-1-3-6(8)13(18)19/h1-4,7,9-12,14-17H,5H2

Clave InChI: InChIKey=SZAOZNVCHHBUDZ-RUXWNWLUSA-N

SMILES: S([C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O)C2=C(N(=O)=O)C=CC=C2

Sinónimos:

• 2-Nitrophenyl 1-Thiohexopyranoside
• 2-Nitrophenyl 1-thio-β-<span class="text-smallcaps">D</span>-galactopyranoside
• 2-Nitrophenyl-β-<span class="text-smallcaps">D</span>-thiogalactopyranoside
• 2-nitrophenyl 1-thio-beta-D-galactopyranoside
• Galactopyranoside, o-nitrophenyl 1-thio-
• Galactopyranoside, o-nitrophenyl 1-thio-, β-<span class="text-smallcaps">D</span>-
• Thio-o-nitrophenyl-β-<span class="text-smallcaps">D</span>-galactopyranoside
• beta-D-Galactopyranoside, 2-nitrophenyl 1-thio-
• o-Nitrophenol beta-thiogalactoside
• o-Nitrophenyl 1-thio-beta-D-galactopyranoside
• o-Nitrophenyl 1-thio-β-<span class="text-smallcaps">D</span>-galactoside
• o-Nitrophenyl β-<span class="text-smallcaps">D</span>-thiogalactoside
• β-<span class="text-smallcaps">D</span>-Galactopyranoside, 2-nitrophenyl 1-thio-
• Thio-o-nitrophenyl-β-D-galactopyranoside
• Galactopyranoside, o-nitrophenyl 1-thio-, β-D-
• 2-Nitrophenyl 1-thio-β-D-galactopyranoside
• β-D-Galactopyranoside, 2-nitrophenyl 1-thio-
• ONP-1-THIO-BETA-D-GAL
• O-NITROPHENYLSULFENYL-1-THIO-BETA-D-GALACTOSIDE
• 2-Nitrophenyl-beta-D-thiogalactopyranoside,98%
• o-Nitrophenylsulfenyl-1-thio-β-D-galactoside
• O-NITROPHENYL-1-THIO-BETA-D-GALACTOSIDE
• 2-Nitrophenylb-D-thiogalactopyranoside
• 1-S-(2-Nitrophenyl)-1-thio-β-D-galactopyranose
• RARECHEM AH BS 0066
• 2-(hydroxymethyl)-6-[(2-nitrophenyl)thio]oxane-3,4,5-triol
• O-NITROPHENYL-1-THIO-BETA-D-GALACTOPYRANOSIDE USP/EP/BP
• O-NITROPHENYL-1-THIO-BETA-D-GALACTOPYRANOSID
• 1-(2-Nitrophenylthio)-1-deoxy-β-D-galactopyranose
• 2-NITROPHENYL-BETA-D-THIOGALACTOPYRANOSIDE
• O-NITROPHENYL-BETA-D-THIOGALACTOPYRANOSIDE
• O-NITROPHENYL 1-THIO-B-D-*GALACTOPYRANOS IDE
• 2-Nitrophenyl β-D-thiogalactopyranoside
• o-Nitrophenyl-1-thio-β-D-galactopyranoside≥ 99% (TLC)

β-D-Galactopyranoside, 2-nitrophenyl 1-thio-

CAS: 1158-17-4

Fórmula: C₁₂H₁₅NO₇S

Pureza: 98%

Forma y color: Solid

Peso molecular: 317.3150

Ref: IN-DA000GWC

2-Nitrophenyl b-D-thiogalactopyranoside

CAS: 1158-17-4

Ref: 7W-GC2622

2-Nitrophenyl 1-thio-β-D-galactopyranoside

CAS: 1158-17-4

2-Nitrophenyl 1-thio-β-D-galactopyranoside

Peso molecular: 317.32g/mol

Ref: 54-BIN1245

2-Nitrophenyl-β-D-thiogalactopyranoside

CAS: 1158-17-4

2-Nitrophenyl-beta-D-thiogalactopyranoside is a chromogenic substrate used to detect the presence of galactosidase enzymes in various biological and biochemical assays. It consists of a nitrophenyl group attached to a galactose sugar molecule through a thioglycosidic bond. When acted upon by enzymes such as galactosidases, it releases the colored nitrophenyl group, which can be detected using spectrophotometry. This substrate is commonly used in assays for the detection of LacZ activity in bacterial cultures, as lacZ encodes for β-galactosidase.

Fórmula: C₁₂H₁₅NO₇S

Pureza: Min. 98 Area-%

Forma y color: Yellow Powder

Peso molecular: 317.32 g/mol

Ref: 3D-EN03315

2-Nitrophenyl-1-thio-β-D-galactopyranoside

CAS: 1158-17-4

2-Nitrophenyl-1-thio-beta-D-galactopyranoside is a nitroaryl compound that can be synthesized from beta-D-galactopyranosides and nitric acid. It has a molecular conformation that is stabilized by hydrogen bonds, which allows it to form supramolecular assemblies with other molecules. The nitro group in this molecule is responsible for the formation of the nitro group in 1,2,4,5,-tetranitrobenzene.

Fórmula: C₁₂H₁₅NO₇S

Peso molecular: 317.32 g/mol

Ref: 3D-N-5100