CAS 142988-22-5

DABCYL-.gamma.-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS

Descripción:

DABCYL-.gamma.-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS es un compuesto químico basado en péptidos que sirve como un sonda fluorescente, comúnmente utilizada en investigación bioquímica y biofísica. La estructura presenta un grupo DABCYL, que actúa como un apagador, y un grupo EDANS, un donante fluorescente, lo que permite aplicaciones de transferencia de energía por resonancia de Förster (FRET). Este compuesto se caracteriza por su secuencia específica de aminoácidos, que incluye varios residuos hidrofóbicos y polares, contribuyendo a su estabilidad y propiedades de interacción en sistemas biológicos. La presencia de DABCYL y EDANS permite el estudio de interacciones de péptidos, cambios conformacionales y actividad enzimática a través de mediciones de fluorescencia. Sus propiedades únicas lo hacen valioso en ensayos para monitorear la actividad proteolítica y otras interacciones moleculares. Además, el diseño del compuesto permite la personalización en aplicaciones de investigación, lo que lo convierte en una herramienta versátil en los campos de la biología molecular y la bioquímica.

Fórmula: C₉₀H₁₂₀N₂₂O₁₆S

InChI: InChI=1/C90H120N22O16S/c1-12-53(7)76(87(122)103-71(43-61-46-94-49-98-61)84(119)107-77(55(9)113)79(115)66-35-34-64-65(80(66)129(126,127)128)22-17-23-67(64)96-38-37-92)106-86(121)75(52(5)6)105-83(118)68(40-51(3)4)100-82(117)70(42-60-45-93-48-97-60)101-81(116)69(41-56-20-15-14-16-21-56)102-85(120)73-24-19-39-111(73)90(125)72(44-62-47-95-50-99-62)104-88(123)78(54(8)13-2)112(74(114)25-18-36-91)89(124)57-26-28-58(29-27-57)108-109-59-30-32-63(33-31-59)110(10)11/h14-17,20-23,26-35,45-55,68-73,75-78,96,113H,12-13,18-19,24-25,36-44,91-92H2,1-11H3,(H,93,97)(H,94,98)(H,95,99)(H,100,117)(H,101,116)(H,102,120)(H,103,122)(H,104,123)(H,105,118)(H,106,121)(H,107,119)(H,126,127,128)

SMILES: CCC(C)C(C(=NC(Cc1cnc[nH]1)C(=NC(C(C)O)C(=O)c1ccc2c(cccc2NCCN)c1S(=O)(=O)O)O)O)N=C(C(C(C)C)N=C(C(CC(C)C)N=C(C(Cc1cnc[nH]1)N=C(C(Cc1ccccc1)N=C(C1CCCN1C(=O)C(Cc1cnc[nH]1)N=C(C(C(C)CC)N(C(=O)CCCN)C(=O)c1ccc(cc1)N=Nc1ccc(cc1)N(C)C)O)O)O)O)O)O

Sinónimos:

• 4-(4-Dimethylaminophenylazo)benzoyl-4-aminobutanoyl-isoleucyl-histidyl-prolyl-phenylalanyl-histidyl-leucyl-valyl-isoleucyl-histidyl-threonyl-5-((2-aminoethyl)amino)naphthalene-1-sulfonic acid
• Dabcyl-GABA-ile-his-pro-phe-his-leu-val-ile-his-thr-edans
• Dabgab-ipfit-edans
• N-(4-((4-((4-(Dimethylamino)phenyl)azo)benzoyl)amino)-1-oxobutyl)-L-isoleucyl-L-histidyl-L-prolyl-L-phenylalanyl-L-histidyl-L-leucyl-L-valyl-L-isoleucyl-L-histidyl-N-(2-((5-sulfo-1-naphthalenyl)amino)ethyl)-L-threoninamide
• L-Threoninamide, N-(4-((4-((4-(dimethylamino)phenyl)azo)benzoyl)amino)-1-oxobutyl)-L-isoleucyl-L-histidyl-L-prolyl-L-phenylalanyl-L-histidyl-L-leucyl-L-valyl-L-isoleucyl-L-histidyl-N-(2-((5-sulfo-1-naphthalenyl)amino)ethyl)-

Renin FRET Substrate I

CAS: 142988-22-5

Renin FRET Substrate I, designed for human renin, contains angiotensinogen's N-terminal cleavage site.

Fórmula: C₉₀H₁₂₀N₂₂O₁₆S

Peso molecular: 1798.15

Ref: TM-T38900

Dabcyl-γ-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Edans

Producto controlado

CAS: 142988-22-5

Dabcyl-γ-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Edans is an angiotensinogen peptide. It has been used as a substrate for Renin and assayed by fluorescence to study the binding affinity of protease inhibitors. Dabcyl is a fluorescent label that can be used in peptide and biochemicals assays, including fluorescence assay. Dabcyl is soluble in water and has little fluorescence quenching with other compounds, making it ideal for use in these applications.

Fórmula: C₉₀H₁₂₀N₂₂O₁₆S

Pureza: Min. 95%

Peso molecular: 1,798.16 g/mol

Ref: 3D-SFQ-3731-PI

DABCYL-gamma-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS trifluoroacetate salt

Producto controlado

CAS: 142988-22-5

DABCYL-gamma-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr (DABCYL) is a fluorescent substrate that has been used to study the kinetics of peptide hydrolysis by proteases. It is an amino acid sequence that is present in angiotensinogen, which is a blood protein involved in regulating blood pressure. The DABCYL group on the terminal amino acid of the peptide provides a highly fluorescent molecule that can be excited at wavelengths longer than 400 nm. This fluorophore can also be used as a donor for fluorescence resonance energy transfer (FRET) with other fluorophores, such as EDANS, which has been shown to have high affinity for DABCYL. DABCYL can be used to measure enzyme activity or inhibition and has been found to be sensitive enough to detect changes due to dilutions at concentrations as low as 10 nM.

Fórmula: C₉₀H₁₂₀N₂₂O₁₆S

Pureza: Min. 95%

Peso molecular: 1,798.12 g/mol

Ref: 3D-FD110909