CAS 28718-90-3

4′,6-Diamidino-2-fenilindol dihidrocloruro

Descripción:

4′,6-Diamidino-2-fenilindol dihidrocloruro (DAPI) es un tinte fluorescente que se une fuertemente a las regiones ricas en A-T en el ADN, lo que lo convierte en una herramienta valiosa en biología molecular y microscopía. Se utiliza comúnmente para teñir núcleos celulares en microscopía de fluorescencia, lo que permite a los investigadores visualizar y cuantificar estructuras celulares. DAPI emite fluorescencia azul cuando es excitado por luz ultravioleta, lo que facilita la identificación de células y sus núcleos en diversas muestras biológicas. El compuesto es soluble en agua y presenta una alta afinidad por el ADN de doble cadena, lo que mejora su utilidad en aplicaciones como el análisis del ciclo celular y estudios de apoptosis. Además, se conoce que DAPI tiene baja toxicidad, lo que lo hace adecuado para la imagenología de células vivas bajo condiciones específicas. Sin embargo, es importante manejar DAPI con cuidado, ya que puede ser un potencial mutágeno. En general, DAPI es un reactivo ampliamente utilizado en los campos de la biología celular, la histología y la genética molecular debido a sus propiedades efectivas de tinción y facilidad de uso.

Fórmula: C₁₆H₁₅N₅·2ClH

InChI: InChI=1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

Clave InChI: InChIKey=FPNZBYLXNYPRLR-UHFFFAOYSA-N

SMILES: C(=N)(N)C=1C=C2C(C=C(N2)C3=CC=C(C(=N)N)C=C3)=CC1.Cl

Sinónimos:

• 1H-Indole-6-carboximidamide, 2-[4-(aminoiminomethyl)phenyl]-, dihydrochloride
• 1H-Indole-6-carboximidamide, 2-[4-(aminoiminomethyl)phenyl]-, hydrochloride (1:2)
• 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide
• 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride
• 2-Phenylindole-4',6-dicarboxamidine dihydrohydrochloride (hydrate)
• 4′,6-Diamidino-2-phenylindole dihydrochloride
• 6-Amidino-2-(4-amidinophenyl)indole dihydrochloride
• Ccris 8836
• FxCycle Violet
• Indole-6-carboxamidine, 2-(p-amidinophenyl)-, dihydrochloride

DAPI 2HCl [for Biochemical Research]

CAS: 28718-90-3

Fórmula: C₁₆H₁₅N₅·2HCl

Pureza: >90.0%(HPLC)

Forma y color: Light yellow to Yellow to Orange powder to crystal

Peso molecular: 350.25

Ref: 3B-A2412

DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]

CAS: 28718-90-3

Fórmula: C₁₆H₁₅N₅·2HCl

Forma y color: Light yellow to Yellow clear liquid

Peso molecular: 350.25

Ref: 3B-D5888

1H-Indole-6-carboximidamide, 2-[4-(aminoiminomethyl)phenyl]-, hydrochloride (1:2)

CAS: 28718-90-3

Fórmula: C₁₆H₁₇Cl₂N₅

Pureza: 97%

Forma y color: Solid

Peso molecular: 350.2457

Ref: IN-DA002W8S

DAPI Dihydrochloride

CAS: 28718-90-3

DAPI Dihydrochloride

Pureza: ≥98%

Peso molecular: 350.24g/mol

Ref: 54-BUP10615

4',6-Diamidino-2-phenylindole dihydrochloride

CAS: 28718-90-3

4',6-Diamidino-2-phenylindole dihydrochloride

Fórmula: C₁₆H₁₅N₅·2ClH

Pureza: 98%

Forma y color: yellow solid

Peso molecular: 350.24568g/mol

Ref: 54-BID0433

4'',6-Diamidino-2-phenylindole dihydrochloride

CAS: 28718-90-3

Fórmula: C₁₆H₁₅N₅·2HCl

Pureza: ≥ 95.0%

Forma y color: Yellow powder

Peso molecular: 350.3

Ref: 7W-GT2414

DAPI Dihydrochloride

CAS: 28718-90-3

DAPI Dihydrochloride (FxCycle Violet) is a cell-permeable fluorescent probe, to bound in groves of A-T-rich DNA sequences. High-Quality, Low-Cost!

Fórmula: C₁₆H₁₇Cl₂N₅

Pureza: 98.83% - 99.94%

Forma y color: Yellow Solid

Peso molecular: 350.24

Ref: TM-T19827

2-(4-CARBAMIMIDOYLPHENYL)-1H-INDOLE-6-CARBOXIMIDAMIDE 2HCL

CAS: 28718-90-3

Pureza: 97.0%

Forma y color: Solid

Peso molecular: 350.25

Ref: 10-F053590

4’,6-Diamidino-2-phenylindole Dihydrochloride

Producto controlado

CAS: 28718-90-3

Stability Hygroscopic
Applications A cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes.
References Jpeeesen, C, and Nielsen, P.E.: Eur. J. Biochem., 182, 437 (1989), Li,J., et al.: J. Exp. Biol., 200, 1499 (1997), Zaitsev, E.N., and Kowalczykowsi, S.C.: Nucl. Acids Res., 26(2), 650 (1998), Germ, M., et al.: Biochem. Biophys. Res. Commun., 261, 452 (1999),

Fórmula: C₁₆H₁₅N₅·2ClH

Forma y color: Yellow Powder

Peso molecular: 350.25

Ref: TR-D416050

4',6-Diamidino-2-phenylindole 2HCl

CAS: 28718-90-3

4',6-Diamidino-2-phenylindole 2HCl (DAB) is a chemical compound used as a histological stain to detect and identify different types of cancer cells. It has been shown to have cytotoxic activity against cancer cells in vitro, but not normal cells. DAB inhibits the growth of cancer cells by disrupting the mitochondrial membrane potential, leading to the release of cytochrome c and apoptosis. The use of this substance has shown that it is not toxic to maternal blood or embryonic tissues. DAB binds to nuclear DNA and polymerase chain reaction products, providing an accurate way to measure cancer cell proliferation.

Fórmula: C₁₆H₁₇Cl₂N₅

Pureza: Min. 97.5 Area-%

Forma y color: Yellow Powder

Peso molecular: 350.25 g/mol

Ref: 3D-FD21444

DAPI Dihydrochloride (4,6-Diamidino-2-Phenylindole Dihydrochloride) for molecular biology, 95%

CAS: 28718-90-3

Fórmula: C₁₆H₁₅N₅·2HCl

Pureza: min. 95%

Forma y color: Light yellow to Yellow to Orange, Powder, Clear, Yellow

Peso molecular: 350.25

Ref: SR-18668

DAPI Dihydrochloride (4,6-Diamidino-2-Phenylindole Dihydrochloride) Solution (1mg/ml in H2O)

CAS: 28718-90-3

Fórmula: ‎C16H15N5.2HCl

Forma y color: Clear, slight pale yellow, Liquid

Peso molecular: 350.25

Ref: SR-80145

DAPI Dihydrochloride (4,6-Diamidino-2-Phenylindole Dihydrochloride) for tissue culture, 95%

CAS: 28718-90-3

Fórmula: C₁₆H₁₅N₅·2HCl

Pureza: min. 95%

Forma y color: Light yellow to Yellow to Orange, Powder, Clear, Yellow

Peso molecular: 350.25

Ref: SR-28488