CAS 35013-72-0

Éster de N-hidroxisuccinimida de biotina

Descripción:

Éster de N-hidroxisuccinimida de biotina, con el número CAS 35013-72-0, es un reactivo de bioconjugación comúnmente utilizado en aplicaciones bioquímicas, particularmente en el etiquetado de proteínas y otras biomoléculas. Este compuesto presenta una parte de biotina, que es una vitamina que desempeña un papel crucial en el metabolismo celular y es conocida por su fuerte afinidad por la avidina y la estreptavidina, lo que la hace invaluable en varios ensayos y técnicas de purificación. El componente N-hidroxisuccinimida (NHS) mejora la reactividad del éster, permitiéndole formar enlaces amida estables con aminas primarias presentes en proteínas o péptidos. Esta característica es particularmente útil para crear proteínas biotiniladas que pueden ser fácilmente aisladas o detectadas. Éster de N-hidroxisuccinimida de biotina es típicamente soluble en disolventes orgánicos y exhibe estabilidad bajo condiciones de almacenamiento apropiadas. Sus aplicaciones se extienden a áreas como la inmunología, la biología molecular y los sistemas de entrega de fármacos, donde el etiquetado y seguimiento precisos de biomoléculas son esenciales para el éxito experimental.

Fórmula: C₁₄H₁₉N₃O₅S

InChI: InChI=1/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1

Clave InChI: InChIKey=YMXHPSHLTSZXKH-RVBZMBCESA-N

SMILES: C(CCCC(ON1C(=O)CCC1=O)=O)[C@H]2[C@@]3([C@@](NC(=O)N3)(CS2)[H])[H]

Sinónimos:

• (+)-Biotinyl-N-hydroxysuccinimide
• (N-Hydroxysuccinimidyl)biotin
• 1-({5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}oxy)pyrrolidine-2,5-dione
• 1-{[5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoyl]oxy}pyrrolidine-2,5-dione
• 1H-Thieno[3,4-d]imidazole, 2,5-pyrrolidinedione deriv.
• 1H-Thieno[3,4-d]imidazole-4-pentanoic acid, hexahydro-2-oxo-, 2,5-dioxo-1-pyrrolidinyl ester, (3aS,4S,6aR)-
• 2,5-Pyrrolidinedione, 1-[[5-(hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl)-1-oxopentyl]oxy]-, [3aS-(3aα,4β,6aα)]-
• 2,5-Pyrrolidinedione, 1-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]oxy]-
• <span class="text-smallcaps">D</span>-Biotin N-hydroxysuccinimide ester
• Biotin N-hydroxysuccinimide
• Biotin N-hydroxysuccinimidyl ester
• Biotin NHS ester
• Biotin succinimidyl ester
• Biotin-Nhs
• Biotin-OSu
• EZ-Link NHS-Biotin
• Iotin-Nhs
• N-Hydroxysuccinimidobiotin
• N-Succinimidyl <span class="text-smallcaps">D</span>-biotinate
• NHS Biotin
• NSC 345668
• Biotin N-hydroxysuccinimide ester
• D-BIOTIN, SE
• (+)-BIOTINYL-N-SUCCINIMIDYL ESTER
• BIOTIN (LONG ARM) NHS
• NHS-D-BIOTIN
• D-BIOTINYL-OSU
• BIOTIN SE
• BNHS
• D-BIOTIN-N-HYDROXYSUCCINIMIDE ESTER
• D-(+)BIOTIN N-HYDROXYSUCCINIMIDE ESTER
• 5-(2-OXO-HEXAHYDRO-THIENO[3,4-D]IMIDAZOL-6-YL)-PENTANOIC ACID 2,5-DIOXO-PYRROLIDIN-1-YL ESTER
• (+)-BIOTIN N-SUCCINIMIDYL ESTER
• BIOTINYL-N-HYDROXY-SUCCINIMIDE

N-Succinimidyl D-Biotinate

CAS: 35013-72-0

Fórmula: C₁₄H₁₉N₃O₅S

Pureza: >97.0%(HPLC)(N)

Forma y color: White to Almost white powder to crystal

Peso molecular: 341.38

Ref: 3B-S0491

(+)-Biotin N-succinimidyl ester, 98%

CAS: 35013-72-0

2,5-dioxopyrrolidin-1-yl 5-{2-oxo-hexahydrothieno[3,4-d]imidazol-4-yl}pentanoate

Fórmula: C₁₄H₁₉N₃O₅S

Pureza: 98%

Forma y color: Powder or crystals or crystalline powder, White to cream

Peso molecular: 341.38

Ref: 02-044771

D-Biotinyl-OSu

CAS: 35013-72-0

BNHS

Fórmula: C₁₄H₁₉N₃O₅S

Pureza: ≥ 97%

Forma y color: White Powder

Peso molecular: 341.39

Ref: 01-4014225

2,5-Pyrrolidinedione,1-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]oxy]-

CAS: 35013-72-0

Fórmula: C₁₄H₁₉N₃O₅S

Pureza: 98%

Forma y color: Solid

Peso molecular: 341.3828

Ref: IN-DA00I6XN

Biotin NHS

CAS: 35013-72-0

Biotin NHS (NHS-Biotin) is an amino reactive biotin reagent used to prepare biotinylated surfaces or polypeptides.

Fórmula: C₁₄H₁₉N₃O₅S

Pureza: 98.27% - 99.15%

Forma y color: Powder

Peso molecular: 341.38

Ref: TM-T14579

Biotin NHS

CAS: 35013-72-0

Biotin NHS

Pureza: ≥98%

Peso molecular: 341.38g/mol

Ref: 54-BUP15883

(+)-Biotin N-hydroxysuccinimide ester

CAS: 35013-72-0

Fórmula: C₁₄H₁₉N₃O₅S

Peso molecular: 341.40

Ref: 7W-GV8230

N-Hydroxysuccinimido biotin

CAS: 35013-72-0

N-Hydroxysuccinimido biotin

Pureza: 98%

Forma y color: White To Off White Powder

Peso molecular: 341.38g/mol

Ref: 54-BIB101

Biotin NHS

Producto controlado

CAS: 35013-72-0

Stability Moisture Sensitive
Applications Amino reactive biotin reagent for preparing biotinylated surfaces or polypeptides.
References Ngo, T.T., et al.: Applied Biochem. Biotech, 7, 443 (1982), Wojchowski, D.M., et al.: J. Immunol. Methods, 90, 173 (1986);

Fórmula: C₁₄H₁₉N₃O₅S

Forma y color: Neat

Peso molecular: 341.38

Ref: TR-S689500

Biotin succinimidyl ester

CAS: 35013-72-0

Biotinylation reagent for labeling nucleic acids or proteins.

Fórmula: C₁₄H₁₉N₃O₅S

Pureza: Min. 98 Area-%

Forma y color: White Off-White Powder

Peso molecular: 341.38 g/mol

Ref: 3D-FB10303

Biotinyl-OSu

CAS: 35013-72-0

Fórmula: C₁₄H₁₉N₃O₅S

Pureza: 95%

Forma y color: Solid, Off-white powder

Peso molecular: 341.38

Ref: 10-F330592

NHS-Biotin

CAS: 35013-72-0

NHS-Biotin is a biotin analog that can be used as an affinity label for the purification of specific proteins. NHS-Biotin is synthesized by reacting monoclonal antibody with lysine residues and a hydroxyl group, which form a covalent bond known as an amide linkage. The NHS-Biotin can then be covalently attached to the protein of interest. This attachment allows the protein to be purified by passing it over a column of immobilized streptavidin. NHS-Biotin has been shown to bind to actin filaments in humans and uptake peptide hormones in the bloodstream. It was also found that human serum contains chemical reactions with NHS-Biotin, forming a disulfide bond between two cysteine residues on the protein.

Fórmula: C₂₈H₃₉F₃N₆O₉

Pureza: Min. 95%

Peso molecular: 660.64 g/mol

Ref: 3D-DPG-5707

Biotin-OSu

CAS: 35013-72-0

Biotin-OSu is a biotin derivative that is used as a fluorescence probe for the detection of DNA binding activity. It has been shown to bind to DNA with high affinity in an experimental model. Biotin-OSu binds to the major groove of DNA and can be used to detect dna binding activities, such as protein-DNA interactions, by forming a ternary complex with the target protein and a fluorophore. This compound has also been shown to react with mouse monoclonal antibodies and human serum proteins, which are transfer reactions. Biotin-OSu has also been used as a coupling agent in peptide hormones and streptavidin polymerase chain reactions (PCR). The presence of disulfide bonds in this compound give it stability but limits its solubility.

Fórmula: C₁₄H₁₉N₃O₅S

Pureza: Min. 95%

Peso molecular: 341.39 g/mol

Ref: 3D-BOS-6003-PI