CAS 76823-03-5

5-carboxifluoresceína

Descripción:

5-carboxifluoresceína es un colorante fluorescente comúnmente utilizado en diversas aplicaciones bioquímicas y analíticas, particularmente en microscopía de fluorescencia y citometría de flujo. Se caracteriza por su brillante fluorescencia verde, que se atribuye a su estructura de xanteno. El compuesto presenta un grupo ácido carboxílico que mejora su solubilidad en soluciones acuosas, lo que lo hace adecuado para estudios biológicos. Su estructura molecular permite una unión efectiva a proteínas y ácidos nucleicos, facilitando su uso como trazador o marcador en imágenes celulares. Además, 5-carboxifluoresceína se emplea a menudo en la síntesis de sondas fluorescentes y en el desarrollo de ensayos para detectar actividad enzimática. El colorante exhibe estabilidad bajo una variedad de condiciones de pH, aunque su fluorescencia puede verse afectada por factores ambientales como el pH y la fuerza iónica. En general, 5-carboxifluoresceína es una herramienta versátil en el campo de la bioquímica y la biología molecular, permitiendo a los investigadores visualizar y cuantificar procesos biológicos con alta sensibilidad.

Fórmula: C₂₁H₁₂O₇

InChI: InChI=1/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)

Clave InChI: InChIKey=NJYVEMPWNAYQQN-UHFFFAOYSA-N

SMILES: O=C1OC2(C=3C1=CC(C(O)=O)=CC3)C=4C(OC=5C2=CC=C(O)C5)=CC(O)=CC4

Sinónimos:

• 3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
• 3′,6′-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylic acid
• 3′,6′-Dihydroxy-3-oxospiro[2-benzofuran-1,9′-xanthene]-5-carboxylic acid
• 3′,6′-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-5-carboxylic acid
• 5-Fam
• Carboxyfluorescein,5-
• FAM (dye)
• Fam 494/520
• Flamma 456 Carboxylic acid
• Hydroxyfluorescein
• Spiro[isobenzofuran-1(3H),9′-[9H]xanthene]-5-carboxylic acid, 3′,6′-dihydroxy-3-oxo-
• 5-Carboxyfluorescein
• FAM
• 3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
• 5-CARBOXYLUORESCEIN
• 5(6)-FAM
• 5-Carboxyfluorescein (5-FAM)
• 4-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-ISOPHTHALIC ACID
• 3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5(6)-carboxylic acid
• 4-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-isophth
• 5-(AND-6)-CARBOXY SNAFL(R)-1
• 4(5)-CARBOXYFLUORESCEIN
• 5-(AND-6)-CARBOXYFLUORESCEIN
• 5-(AND-6)-CARBOXY SNARF(R)-1
• 4-carboxyfluorescein
• 5-(AND-6)-CARBOXY SNAFL(R)-2
• 5-CF

5-Carboxyfluorescein

CAS: 76823-03-5

Fórmula: C₂₁H₁₂O₇

Pureza: >97.0%(T)(HPLC)

Forma y color: Light yellow to Brown powder to crystal

Peso molecular: 376.32

Ref: 3B-C2477

5-Carboxyfluorescein, single isomer, 97%

CAS: 76823-03-5

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Sci

Fórmula: C₂₁H₁₂O₇

Pureza: 97%

Forma y color: Powder or crystals or crystalline powder, Yellow to orange

Peso molecular: 376.32

Ref: 02-H66949

5-Carboxy-fluorescein

CAS: 76823-03-5

Widely used green-fluorescent labeling dye.

Fórmula: C₂₁H₁₂O₇

Pureza: > 98%

Forma y color: Yellow Powder

Peso molecular: 376.32

Ref: 01-4045141

5-FAM

CAS: 76823-03-5

5-FAM, a carboxylic acid, reacts with amines for biomolecule labeling; excites at 488 nm, high fluorescence, solubility, absorbance.

Fórmula: C₂₁H₁₂O₇

Pureza: 98.28% - 99.32%

Forma y color: Orange Solid

Peso molecular: 376.32

Ref: TM-T14050

3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid

CAS: 76823-03-5

Fórmula: C₂₁H₁₂O₇

Pureza: 97%

Forma y color: Solid

Peso molecular: 376.3158

Ref: IN-DA008P3R

5-Carboxyfluorescein

CAS: 76823-03-5

Fórmula: C₂₁H₁₂O₇

Pureza: ≥ 99.0%

Forma y color: Yellow to orange powder

Peso molecular: 376.3

Ref: 7W-GT3783

5-FAM

CAS: 76823-03-5

5-FAM

Pureza: ≥98%

Peso molecular: 376.32g/mol

Ref: 54-BUP14627

5-Carboxyfluorescein

CAS: 76823-03-5

5-Carboxyfluorescein

Pureza: 98%

Peso molecular: 376.32g/mol

Ref: 54-BIC1050

5-Carboxyfluorescein (Technical Grade)

Producto controlado

CAS: 76823-03-5

Applications 5-Carboxyfluorescein is a useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent. Dyes and metabolites.
References Theisen, P., et al.: Tetrahedron Lett., 33, 5033 (1992); Mattingly, P.: Bioconjgate Chem., 3, 430 (1992); Bertrand, R., et al.: Biochemistry, 34, 9500 (1995);

Fórmula: C₂₁H₁₂O₇

Forma y color: Yellow To Dark Yellow

Peso molecular: 376.32

Ref: TR-C178320

5-Carboxyfluorescein

CAS: 76823-03-5

5-Carboxyfluorescein (5-FAM) is a self-quenching dye and one of the most common labelling agents for biomolecules, cell staining and localisation studies. The fluorescence is pH dependent, therefore it has been used to measure the internal pH of cells. As it is only membrane permeable to dead cells, it can be used as a staining agent to distinguish viable and non-viable cells. It is also used in PCR to label the 5’ end of oligonucleotides in the presence of a quencher at the 3’ end (dual labelled probe). During the amplification process, the dye is cleaved and the fluorescence increases proportionally with the amount of the specific sequence amplified during the PCR process. The development of the fluorescence signal is therefore specifically related to the amplification of the target sequence. The carboxylic acid can be activated to react with a primary amine.

Fórmula: C₂₁H₁₂O₇

Pureza: Min. 98 Area-%

Forma y color: Yellow Powder

Peso molecular: 376.32 g/mol

Ref: 3D-FC19773

3′,6′-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylic acid

CAS: 76823-03-5

Fórmula: C₂₁H₁₂O₇

Pureza: 95%

Forma y color: Solid

Peso molecular: 376.32

Ref: 10-F448180

5-Carboxyfluorescein

CAS: 76823-03-5

Fórmula: C₂₁H₁₂O₇

Peso molecular: 376.32

Ref: ST-EA-CP-F116029