CAS 85901-57-1

Pyr-Phe-Leu-pNA

Descripción:

Pyr-Phe-Leu-pNA, también conocido como Leucina p-Nitroanilida de Fenilo Piridilo, es un sustrato peptídico sintético comúnmente utilizado en ensayos bioquímicos, particularmente en el estudio de enzimas proteolíticas. Este compuesto presenta un grupo piridina, un residuo de fenilalanina, un residuo de leucina y un moiety de p-nitroanilida, que en conjunto contribuyen a sus propiedades como sustrato para varias proteasas. La presencia del grupo p-nitroanilida permite la detección colorimétrica tras la escisión por enzimas, lo que lo hace útil en ensayos de actividad enzimática. Pyr-Phe-Leu-pNA es típicamente soluble en disolventes orgánicos y exhibe estabilidad bajo condiciones de laboratorio estándar. Su estructura molecular permite interacciones específicas con enzimas objetivo, facilitando el estudio de la cinética y mecanismos enzimáticos. Además, debido a su naturaleza sintética, puede ser modificado para mejorar la especificidad o sensibilidad para aplicaciones particulares en investigación y diagnóstico. Al igual que con todas las sustancias químicas, se deben observar las precauciones adecuadas de manejo y seguridad al trabajar con Pyr-Phe-Leu-pNA en entornos de laboratorio.

Fórmula: C₂₆H₃₁N₅O₆

InChI: InChI=1/C26H31N5O6/c1-16(2)14-21(25(34)27-18-8-10-19(11-9-18)31(36)37)29-26(35)22(15-17-6-4-3-5-7-17)30-24(33)20-12-13-23(32)28-20/h3-11,16,20-22H,12-15H2,1-2H3,(H,27,34)(H,28,32)(H,29,35)(H,30,33)/t20-,21-,22-/m0/s1

SMILES: CC(C)C[C@@H](C(=O)Nc1ccc(cc1)N(=O)=O)N=C([C@H](Cc1ccccc1)N=C([C@@H]1CCC(=N1)O)O)O

Sinónimos:

• Pyroglutamyl-phenylalanyl-leucine-4-nitroanilide
• Pglu-phe-leu-pna
• L-Leucinamide, 5-oxo-L-prolyl-L-phenylalanyl-N-(4-nitrophenyl)-
• 5-oxo-L-prolyl-L-phenylalanyl-N-(4-nitrophenyl)-L-leucinamide
• pglu-phe-leu P-nitroanilide
• PYR-FL-PNA
• PYR-PHE-LEU-PNA
• PYR-PHE-GLY-PNA
• Pyr-FG-pNA
• L-PYROGLUTAMYL-L-PHENYLALANYL-L-LEUCINE P-NITROANILIDE

Pyr-Phe-Leu-pNA

CAS: 85901-57-1

Chromogenic substrate for thiol proteinases from plants (caricain, papain, ficin, and bromelain) and from mammalian brain (cathepsin B). pEFL-pNA is also cleaved by a serine carboxypeptidase (HlSCP1) identified in the midgut of the hard tick Haemaphysalis longicornis, which was shown to process hemoglobin.

Fórmula: C₂₆H₃₁N₅O₆

Pureza: 99.4%

Forma y color: Light Yellow Powder

Peso molecular: 509.56

Ref: 01-4010747

Pyr-Phe-Leu-pNA

CAS: 85901-57-1

Pyr-Phe-Leu-pNA is a proteolytic enzyme that can be used as a pharmaceutical drug. It has been shown to have insecticidal activity against the Anopheles mosquito and other insects, as well as antimicrobial activity against bacteria and fungi. Pyr-Phe-Leu-pNA is activated by tryptophan fluorescence, which is why it is suitable for use in peptide mapping. This enzyme also has an important physiological function in the zymogen activation pathway, which enables it to act as an enzyme substrate. Pyr-Phe-Leu-pNA will also degrade proteins that contain sequences of leishmania protease activity or signal peptide.

Fórmula: C₂₆H₃₁N₅O₆

Pureza: Min. 95%

Peso molecular: 509.55 g/mol

Ref: 3D-SPL-3130

Pyr-Phe-Leu-pNA

CAS: 85901-57-1

Pyr-Phe-Leu-pNA is a proteolytic enzyme that is used in the production of monoclonal antibodies. The enzyme was originally isolated from human pancreas, but has also been found in other sources including eggs, bovine pancreas, and various bacteria. Pyr-Phe-Leu-pNA hydrolyzes peptide bonds with a preference for serine and threonine residues. This enzyme has been shown to be effective at cleaving influenza virus protein hemagglutinin, which may be useful in the development of new vaccines. Pyr-Phe-Leu-pNA has also been shown to have high salt tolerance, making it a good candidate for use in food processing applications.

Fórmula: C₂₆H₃₁N₅O₆

Pureza: Min. 95%

Peso molecular: 509.55 g/mol

Ref: 3D-FP110746