Sustratos fluorogénicos
En esta categoría, encontrará una selección completa de sustratos fluorogénicos diseñados para la catálisis enzimática. Estos sustratos se utilizan con varios tipos de enzimas y son capaces de producir color y fluorescencia al reaccionar. Los sustratos fluorogénicos son herramientas esenciales en la investigación bioquímica y biología molecular, permitiendo la detección y cuantificación de la actividad enzimática con alta sensibilidad y especificidad. Se utilizan ampliamente en ensayos para estudiar la cinética enzimática, la búsqueda de inhibidores enzimáticos y el monitoreo de procesos celulares. En CymitQuimica, ofrecemos sustratos fluorogénicos de alta calidad para apoyar su investigación, asegurando resultados fiables y precisos en sus ensayos enzimáticos.
Se han encontrado 297 productos de "Sustratos fluorogénicos"
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FLsharp™ - Phosphate, disodium salt
FLsharp™ - Phosphate, disodium salt is a fluorogenic water-soluble substrate for phosphatase enzymes. Its chemical name is 6-Chloro-2-[5-chloro-2-(phosphonooxy)phenyl]-4(1H)-quinazolinone disodium salt and was developed by Molecular Probes in the 1990s. After cleavage of the substrate by phosphatase activity, an insoluble product is formed that is highly fluorescent in the solid state. Upon UV irradiation this very stable fluorophore emits green light in the 530 nm range for an extended period of time. FLsharp™ is a unique substrate - optimized for the detection of phosphatase activity in solid matrices such as agar media, tissue sections or microscopic preparations. FLsharp™ - Phosphate, disodium salt is used in microbiology as a substrate for bacterial phosphatases.
Pureza:Min. 95%4-Methylumbelliferyl beta-D-lactoside
CAS:Fluorogenic substrate for beta-galactosidaseFórmula:C22H28O13Pureza:Min. 98 Area-%Forma y color:White PowderPeso molecular:500.45 g/mol4-Methylumbelliferyl oleate
CAS:Methylumbelliferyl oleate is a MOSCERDAM® substrate targeting the lipase enzymeFórmula:C28H40O4Pureza:Min. 90 Area-%Forma y color:White PowderPeso molecular:440.61 g/mol4-Methylumbelliferyl pyrophosphate diester disodium salt
CAS:4-Methylumbelliferyl pyrophosphate diester disodium salt is a fluorogenic substrate for pyrophosphatases or diphosphatases. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl pyrophosphate diester disodium saltas a substrate for measuring the pyrophosphatases or diphosphatases activity is used for monitoring and diagnosis of pyrophosphatase deficiency and bone metabolism disorder.Fórmula:C20H14Na2O11P2Pureza:Min. 95%Forma y color:White PowderPeso molecular:538.25 g/mol4-(Trifluoromethyl)umbelliferyl-β-D-cellotrioside
CAS:4-(Trifluoromethyl)umbelliferyl-beta-D-cellotrioside is a fluorogenic substrate for cellulase. After cleavage, the fluorophore 4-(trifluoromethyl)umbelliferone, commonly referred as TFMU, is released, exhibiting a blueish color. 4-(Trifluoromethyl)umbelliferyl-beta-D-cellotrioside is used to study the enzyme kinetics in bacterial cellulose degradation assays as well as in biomass conversion research studies.Fórmula:C28H35F3O18Pureza:Min. 95%Forma y color:White to off-white solid.Peso molecular:716.56 g/mol4-Methylumbelliferyl b-L-fucopyranoside
CAS:A MOSCERDAM® substrate targeting the fucosidase enzymeFórmula:C16H18O7Pureza:Min. 98 Area-%Forma y color:PowderPeso molecular:322.31 g/mol4-Methylumbelliferyl-2-O-(α-L-fucopyranosyl)-β-L-fucopyranoside
4-Methylumbelliferyl-2-O-(alpha-L-fucopyranosyl)-beta-L-fucopyranoside is a fluorogenic substrate for alpha-L-fucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl-2-O-(alpha-L-fucopyranosyl)-beta-L-fucopyranosideas a substrate for measuring the alpha-L-fucosidase activity is used for diagnosis of fucosidosis (defficiency of L-fucosidase).Pureza:Min. 95%Forma y color:White/Off-White SolidPeso molecular:468.45 g/molResorufin b-D-xylobioside
Resorufin b-D-xylobioside is a fluorogenic substrate for xylanases useful for xylanase activity assays.Pureza:Min. 95%Forma y color:PowderPeso molecular:477.42 g/mol4-Methylumbelliferyl dodecanoate
CAS:4-Methylumbelliferyl dodecanoate is a fluorogenic substrate for lipase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl dodecanoateas a substrate for measuring the lipase activity is used for detecting pancreatic insufficiency in diagnosis assays as well as the screening of lipase deficiency.Fórmula:C22H30O4Pureza:Min. 95%Forma y color:PowderPeso molecular:358.47 g/mol4-Methylumbelliferyl a-L-idopyranosiduronic acid 2-sulphate disodium salt - Moscerdam™ biochemical purity
CAS:PAIRED PRODUCT AVAILABLE: alpha-L-iduronidase Enzyme, FI1793834MU-alpha-L-iduronide-2-sulphate (M2) is a fluorogenic substrate of iduronate-2-sulphatase (IDS), used in a 2-step assay coupled with alpha-L-iduronidase. The assay is used in biochemical testing for Hunter syndrome (mucopolysaccharidosis II / MPS II). In a first step, IDS from biological samples cleaves the sulphate group from M2. In a second step, exogeneous alpha-L-iduronidase which cleaves the 4-MU moiety is added to the reaction, resulting in release of a fluorescent signal (Ex 360 nm / Em 455 nm). The reaction is stopped by the addition of sodium carbonate.Fórmula:C16H14Na2O12SPureza:Min. 99 Area-%Forma y color:White PowderPeso molecular:476.32 g/mol4-Methylumbelliferyl a-D-glucosaminide
CAS:4-Methylumbelliferyl alpha-D-glucosaminide is a fluorogenic substrate for alpha-N-acetylglucosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl alpha-D-glucosaminideas a substrate for measuring the alpha-N-acetylglucosaminidase activity is used for Sanfilippo syndrome B and Mucopolysaccharidosis IIIB (MPS IIIB) diagnosis.Fórmula:C16H19NO7Pureza:Min. 90 Area-%Forma y color:White PowderPeso molecular:337.32 g/molL-Alanine-7-amido-4-methylcoumarin hydrochloride
CAS:L-Alanine-7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for alanine aminopeptidase.Fórmula:C13H14N2O3·HClPureza:Min. 90%Forma y color:PowderPeso molecular:282.72 g/mol4-Methylumbelliferyl 2-acetamido-3-O-(b-D-galactopyranosyl)-a-D-galactopyranoside
CAS:4-Methylumbelliferyl 2-acetamido-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside is a fluorogenic substrate for alpha-N-acetylgalactosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranosideas a substrate for measuring the alpha-N-acetylgalactosaminidase activity is used for Fabry disease diagnosis and Schindler disease screening.Fórmula:C24H31NO13Pureza:Min. 95%Forma y color:White PowderPeso molecular:541.5 g/mol(+)-Biotin 4-amidobenzoic acid sodium salt
CAS:(+)-Biotin 4-amidobenzoic acid sodium salt, often referred as (+)-biotin PABA, is a fluorogenic substrate used to measure biotinidase activity, especially in newborn screening programs.
Fórmula:C17H20N3NaO4SPureza:Min. 95%Forma y color:White Slightly Yellow PowderPeso molecular:385.41 g/mol4-Methylumbelliferyl a-L-idopyranosiduronic acid sodium salt
CAS:PAIRED PRODUCT AVAILABLE: alpha-L-iduronidase Enzyme, FI179383.
4MU-alpha-L-idopyranosiduronic acid is a fluorogenic substrate for alpha-L-iduronidase. Active alpha-L-iduronidase cleaves the MU moiety from the synthetic substrate, which results in release of a fluorescent signal (Ex 360 nm / Em 455 nm). The reaction is stopped by the addition of sodium carbonate. 4MU-alpha-L-idopyranosiduronic acid is used in alpha-L-iduronidase assays in the context of biochemical characterisation of samples from patients affected by lysosomal storage disease mucopolysaccharidosis I (MPS I).Fórmula:C16H15NaO9Pureza:Min. 98 Area-%Forma y color:White PowderPeso molecular:374.27 g/mol4-Methylumbelliferyl palmitate
CAS:4-Methylumbelliferyl palmitate, commonly names as 4-MUP, is a fluorescent probe that binds to lipases, enzymes responsible to assist on the hydrolysis of fats. In diagnosis, 4-MUP is used for screening of lysosomal storage diseases (LSD) caused by a deficiency of a single enzyme involved in the metabolism of glycoproteins, lipids or mucopolysaccharides.
Fórmula:C26H38O4Pureza:Min. 95%Forma y color:White PowderPeso molecular:414.58 g/molResorufin b-D-glucuronide sodium salt
CAS:Resorufin b-D-glucuronide sodium salt is used as a fluorogenic substrate for beta-glucuronidase (GUS) to study drug metabolism.Pureza:Min. 95%Forma y color:Brown Orange Red PowderPeso molecular:411.29 g/mol4-Methylumbelliferyl 2-acetamido-2-deoxy-b-D-galactopyranoside-4-sulfate sodium salt
4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-galactopyranoside-4-sulfate sodium salt is a fluorogenic substrate for N-acetylgalactosamine-4-sulfatase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-galactopyranoside-4-sulfate sodium saltas a substrate for measuring the N-acetylgalactosamine-4-sulfatase activity is used for diagnosis of Mucopolysaccharidosis VI (MPS VI) (a rare lysosomal storage disorder) and Maroteaux-Lamy syndrome screening.Pureza:Min. 95%Forma y color:White PowderPeso molecular:481.41 g/mol6-Hexadecanoylamino-4-methylumbelliferone
CAS:Fluorochrome for calibration of fluorimeters. Used with corresponding fluorogenic substrates for the diagnosis of Krabbe - and Niemann-Pick A/B disease.Fórmula:C26H39NO4Pureza:Min. 95%Forma y color:White PowderPeso molecular:429.59 g/molFluorescein mono-b-D-galactopyranoside
CAS:Fluorescein mono-b-D-galactopyranoside or FMG is a fluorogenic substrate for beta-galactosidase. The fluorophore fluorescein is released after enzymatic cleavage, exhibiting a greenish fluorescent. This substrate is particularly valuable in gene expression studies (e.g., lacZ reporter assays) and as a probe for cell-based assays for real-time monitoring.Pureza:Min. 95%Forma y color:PowderPeso molecular:494.45 g/mol
