Sustratos fluorogénicos

<p>Colchicine fluorescein is the fluorogenic form of colchicine. It is used for staining assembly of tubulin. Colchicine-site inhibitors (abbrevated as CBSIs) disrupt tubulin polymerization. Colchicine fluorescein serves as a probe to study the binding of colchicine to tubulin, as the colchicine binding site is a promising target for discovering novel antitumour agents.</p>

<p>6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-cellotetraoside is a fluorescent substrate that is used to measure the activity of the enzyme beta-glucuronidase. It is a chemiluminescent compound that emits light when hydrolyzed by an enzyme. 6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-cellotetraoside has been shown to be a high quality and environmentally friendly product, as it does not cause any significant change in pH or turbidity in water samples. It has also been shown to be nontoxic and nonmutagenic at concentrations up to 400 ppm. This product can be used for a variety of purposes, including diagnostic tests for bacterial infections or food testing.</p>

<p>4-Methylumbelliferyl 2-O-(2,3,4-tri-O-benzoyl-a-L-fucopyranosyl)-b-D-galactopyranoside is a fluorescent substrate for β–galactosidase. It is used to detect the enzyme in biological samples and as a diagnostic agent. This substrate can be used to develop chemiluminescent, chromogenic, and fluorogenic substrates for use in diagnostics. 4MUF 2OBG can also be conjugated to various ligands for use in bioluminescence assays or as a marker in immunoassays.</p>

<p>4-Methylumbelliferyl 3,4,6-tri-O-acetyl-2-deoxy-2-trifluoroacetamido-a-D-glucopyranoside (4MUAA) is a fluorescent dye that is used for the detection of envelopment in cells. This dye binds to cell membranes and can be detected with a fluorescence microscope. 4MUAA can be used to determine the efficiency of an experimental methodology or algorithm by measuring the change in fluorescence intensity as a result of experimental treatment. The use of 4MUAA has been shown to be effective in determining whether or not there is an association between nationwide environmental pollution and cancer rates in Japan.</p>

<p>4-Methylumbelliferyl alpha-L-idopyranoside is a fluorogenic substrate for alpha-L-iduronidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl alpha-L-idopyranosideas a substrate for measuring the alpha-L-iduronidase activity is used for Mucopolysaccharidosis I diagnosis and Hurler syndrome screening.</p>

<p>4-Trifluoromethylumbelliferyl Tetra-O-acetylated alpha-D-N-Acetylneuraminate Methyl Ester is a chemiluminescent substrate that is often used in enzyme and immunoassays. It is a high purity, high quality chemical with a CAS number of 195210-09-4. The 4-Trifluoromethylumbelliferyl Tetra-O-acetylated alpha-D-N-Acetylneuraminate Methyl Ester is used in the detection of bacterial infections and can be used to measure the activity of enzymes such as lysozyme and peroxidase. This product has been tested for its ability to induce bioluminescence in bacteria grown in culture media, as well as for its ability to cause fluorescence or staining in cells or tissues. The 4TUMBTM Tetra O Acetylated</p>

<p>6-Chloro-4-methylumbelliferyl b-xylobioside is a fluorogenic substrate that can be used for food testing, environmental testing and diagnostics. It is a chromogenic substrate that can be used in enzyme substrates. 6-Chloro-4-methylumbelliferyl b-xylobioside is also a conjugate and has high purity. This product has chemiluminescent properties and can be used for bioluminescence. It is an excellent ligand for fluorescence labeling and has been shown to have high quality. This product can be used in culture media as well as to detect the presence of bacteria.</p>

<p>Resorufin phosphate disodium is a fluorogenic substrate for phosphatase exhibiting a red color after cleavage that can be measured at ~ 585nm.</p>

<p>Beta-alanine 7-amido-4-methylcoumarin, trifluoroacetate salt is a high purity, ligand, and conjugate. It can be used in the food testing and environmental testing fields. This product has been shown to be a good substrate for enzyme activity and can also function as a fluorescent dye in chemiluminescence assays. Beta-alanine 7-amido-4-methylcoumarin, trifluoroacetate salt is soluble in water and has a CAS number of 201847-54-3.</p>

<p>6-Chloro-7-hydroxy-4-(trifluoromethyl)coumarin is a fungicide that inhibits the growth of fungi by interfering with their DNA synthesis. It does this by inhibiting the activity of two enzymes: the fungal enzyme oxime and the enzyme cinerea, which is found in many fungi. 6-Chloro-7-hydroxy-4-(trifluoromethyl)coumarin has been shown to be effective against Alternaria and Botrytis cinerea. The compound also has an antifungal effect against other types of fungi, such as Penicillium roqueforti, when used in combination with a solvent such as ether.</p>

<p>For fluorogenic detection of caspase 3</p>

<p>Resorufin ethyl ether is a fluorescent substrate that is used to measure the activity of cytochrome P450 enzymes. It can be used in vitro to study the effects of drugs on enzyme activities and in vivo to study liver lesions. Resorufin ethyl ether is also used as a model system for studying synchronous fluorescence, which is the emission of light by two or more substances at the same time. The nitrogen atoms in resorufin ethyl ether can be substituted with other atoms such as magnesium or zinc, which will affect its activity and fluorescence properties. Resorufin ethyl ether binds to cytochrome P450 enzymes and therefore inhibits their activity. This inhibition is reversible, and it can be reversed by adding a substance that competes with the substrate for binding sites on the enzyme.</p>

<p>4-Chloromethyl-6,8-difluoroumbelliferyl b-D-galactopyranoside is a compound commonly used in biochemical research as a fluorescent substrate for the detection of sugar hydrolase enzymes. This compound can be cleaved by enzymes such as β-galactosidase, releasing a fluorescent molecule that can be easily detected using fluorescence spectroscopy. The compound is a member of the family of umbelliferyl glycosides, which have been widely used in the study of various biochemical pathways for many years. The advantage of using this particular substrate is that the fluorescent product has a high quantum yield, which means it produces a strong signal for detection. This substrate has been used in a wide variety of applications, including the study of microbial physiology and in the development of diagnostic assays for detecting bacterial and fungal infections.</p>

<p>2-Dodecylresorufin is a fluorescent probe that reacts with the hydroxyl radicals in the cell, which are generated by the enzyme superoxide dismutase. 2-Dodecylresorufin can be used to measure reactive oxygen species (ROS) and ROS-induced cellular damage. It is also used to study oxidative stress in vitro. The chemical structure of 2-dodecylresorufin contains a linker arm that provides a reversible linkage to the cell surface, allowing for extracellular measurements of reactive oxygen species (ROS). This compound has been shown to have high reactivity, making it useful for studying population dynamics and fluorescent techniques.</p>

<p>Green fluorescent cationic dye</p>

<p>4-Methylumbelliferyl 3,4,6-tri-O-acetyl-2-O-(2,3,4-tri-O-benzoyl-a-L-fucopyranosyl)-b-D-galactopyranoside is a fluorogenic substrate that is used for the detection of β D -galactosidase. β D -Galactosidase is an enzyme that breaks down lactose in milk and is used as a diagnostic tool for identifying lactose intolerance. This substrate can be used as a conjugate in immunoassays to detect antibodies against the enzyme or it can be used to measure the activity of the enzyme in food samples. It also can be used to measure α1,3 galactosyltransferase activity in colorectal cancer cells or bacteria.</p>

<p>5-Acetamidofluorescein-di-(b-D-galactopyranoside), also known as C2FDG, is a fluorogenic substrate for beta-galactosidase that generates a green fluorescence after cleavage (Em ~520 nm). It is used in enzyme activity assays and in flow cytometry in animal, bacterial, and yeast cells.</p>

<p>6,8-Difluoro-4-methylumbelliferyl b-D-glucopyranoside is a versatile fluorogenic substrate suitable for the measurement of various glycosidase enzymes. Its unique structure allows for enhanced sensitivity and selectivity in enzyme assays, making it ideal for studying enzyme kinetics and screening of potential enzyme inhibitors.</p>

<p>4-Methylumbelliferyl 4-deoxy-b-D-chitobiose peracetate is a chromogenic substrate used for the detection of β-galactosidase activity. It is an analog of 4-methylumbelliferone that can be hydrolyzed by β-galactosidase to produce fluorescence. This product is supplied as a white crystalline solid and can be stored at room temperature. This substrate has been shown to work well in culture media, food testing, and environmental testing.</p>

<p>Maltotetraose 2-aminobenzamide is a chemiluminescent substrate that emits light in the presence of certain oxidizing agents. It provides high quality and environmental testing, as well as staining and ligand capability. Maltotetraose 2-aminobenzamide is used in diagnostics to identify bacteria and other microorganisms. Maltotetraose 2-aminobenzamide can be used for the detection of bacterial colonies on agar plates using a fluorogenic substrate, or for the detection of fungi using a chromogenic substrate. Maltotetraose 2-aminobenzamide is also used as an enzyme substrate in food testing or conjugate with enzymes for bioluminescence.</p>