Sustratos fluorogénicos

This Clips-O substrate 7-(4'-L-Alaninamido)-rac-(3'-octanoyloxy)butyloxy) coumarin allows the detection of three enzymatic activities (L-alanine aminopeptidase, C8-esterase and oxidation of 4-amino-3-hydroxybutyloxy either enzymatically or by exposure to sodium periodate) with one single substrate and give a positive fluorescent signal only in presence of all activities. The main virtues of Clips-O substrates are an increased stability towards non-enzymatic hydrolysis that results in a reduced background and high sensitivity.

6,8-Difluoro-4-methylumbelliferyl b-D-glucopyranoside is a versatile fluorogenic substrate suitable for the measurement of various glycosidase enzymes. Its unique structure allows for enhanced sensitivity and selectivity in enzyme assays, making it ideal for studying enzyme kinetics and screening of potential enzyme inhibitors.

4-Methylumbelliferyl 3,4,6-tri-O-acetyl-2-deoxy-2-trifluoroacetamido-b-D-glucopyranoside is a fluorogenic substrate specifically designed for the detection and quantification of hexosaminidase enzymes. Upon cleavage by the target enzyme, it generates a bright fluorescent signal facilitating the investigation of enzyme activity, screening of inhibitors, and diagnostics.

4-Methylumbelliferyl-phosphoethanolamine is a phosphorylated substrate that can be used in the detection of enzymes such as alkaline phosphatase, esterase, and lipase. This compound has been shown to be a fluorescent and chromogenic substrate for many diagnostic purposes. 4-Methylumbelliferyl-phosphoethanolamine is also used as a fluorogenic substrate for chemiluminescent reactions that are found in culture media and environmental testing.

3-Carboxyumbelliferyl b-D-galactopyranoside is a fluorescent probe that can be used to study protein synthesis in live cells. It binds to the active site of the enzyme peptidyl transferase, which catalyzes the covalent linkage of amino acids to form a polypeptide chain. 3CMBG is specific for the amino acid proline and is a substrate for peptidyl transferase. The fluorescence of 3CMBG increases when it is bound to peptidyl transferase, making it useful as a marker for protein synthesis.

4-Methylumbelliferyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-a-D-glucopyranoside is a chromogenic substrate for enzymes that catalyze the hydrolysis of glycosidic bonds. It can be used in diagnostic tests to identify antibodies against certain bacteria and fungi. The fluorogenic form of 4-methylumbelliferyl 2-acetamido-3,4,6-triO acetyl -2 deoxy a D glucopyranoside can be used as a chemiluminescent substrate for detecting bacterial activity. 4 methylumbelliferyl 2 acetamido 3,4,6 triO acetyl -2 deoxy a D glucopyranoside is also useful in bioluminescent assays to detect ATP production in bacteria and other microorganisms. CAS No: 1174287–76–3

4-Methylumbelliferyl-b-D-xylotrioside is a fluorogenic substrate that reacts with an enzyme in the presence of oxygen to produce light. It is used in diagnostic applications, such as detecting the presence of a specific enzyme or protein. 4-Methylumbelliferyl-b-D-xylotrioside can also be used as a chromogenic substrate for the detection of DNA and RNA. This compound can be conjugated with other molecules for use in immunoassays and other diagnostic applications. 4-Methylumbelliferyl-b-D-xylotrioside has been shown to bind to bacterial cells and activate them, which leads to the production of light through bioluminescence.

For fluorogenic detection of caspase 3

4-Trifluoromethylumbelliferyl a-D-N-acetylneuraminic acid is a fluorescent chemiluminescent substrate that is used in the detection of beta-galactosidase activity. Beta-galactosidase catalyzes the hydrolysis of 4-trifluoromethylumbelliferyl a-D-N-acetylneuraminic acid to form 4-trifluoromethylumbelliferone and D-galactose, which emits light at approximately 480 nm. This reaction can be detected by fluorescence spectrophotometry using a fluorometer or photometer. 4TFUNA is not toxic, does not inhibit enzymes, and has been tested for toxicity and mutagenicity using bacteria and mammalian cells.

4-Methylumbelliferyl 2-acetamido-3-O-(2,3,4-tri-O-acetyl-a-L-fucopyranosyl)-4-O-(2,3,4,6 -tetra-O-acetylbDgalactopyranosyl)-6-O-acetyldEoxybDglucopyranoside is a chemiluminescent substrate that has been used in the detection of beta galactosidase activity. It is a ligand for beta galactosidases and can be used for the study of enzyme kinetics. This compound also exhibits high purity and is available in CAS number: 916071. 4MUFAGAALF is suitable for use in culture media and environmental testing as well as diagnostics (e.g., bioluminescence), staining (fluorogenic substrate), and chromogenic substrates

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4-Methylumbelliferone is a fluorescent probe that can be used to study the metabolism of fatty acids in plants. It is also used as a marker for radiation and significant cytotoxicity. 4-Methylumbelliferone binds to the DNA termini, specifically at the 5' phosphate group, and inhibits phosphatase activity. This process increases levels of p-nitrophenyl phosphate (a substrate), which produces protocatechuic acid (an intermediate). The disulfide bond is then broken by hydrogen peroxide, leading to an increase in fluorescence. The optimum pH for this process is 7.5.

Boc-Val-Pro-Arg-PAB-Resorufin is fluorogenic substrate for thrombin and the thrombin-staphylocoagulase complex.

4-Methylumbelliferyl beta;-D-glucuronide (MUG) is commonly used as a substrate for detecting beta;-glucuronidase. MUG is used for identifying E. coli contamination in food, drinkingwater, clinical samples and for rapid bacterial identification in blood cultures. It is recommended in international normatives (ISO, DIN) as a fluorogenic substrate for detection of E. coli. MUG is also a tool assaying the glucuronidase gene in plants.

Fluorogenic substrate for β-galactosidase

Please enquire for more information about 4-Methylumbelliferyl 2-acetamido-2-deoxy-b-D-galactopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page

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4-Methylumbelliferyl alpha-L-idopyranosiduronic acid 2-sulfate disodium salt is a chromogenic substrate that is used in the detection of glycosidases. It reacts with enzymes such as alpha-amylase, beta-galactosidase, and beta-glucosidase to produce a fluorescent product. This product can be detected using a fluorometer or spectrophotometer. The CAS number for 4-methylumbelliferyl alpha-L-idopyranosiduronic acid 2-sulfate disodium salt is 1045020-74-3.

Bis(4-methylumbelliferyl)phosphate is a fluorescent probe that can be used for the diagnosis of β-glucosidase. It has a phosphate group and can be used to study phosphodiesterases and phosphodiesters. Bis(4-methylumbelliferyl)phosphate is also a liposomal probe that can be used in kinetic studies. This compound has been shown to inhibit β-glucosidase, which is an enzyme found in human saliva and pancreatic juice, at high concentrations. The inhibition of this enzyme leads to the accumulation of glucose and galactose in the blood, which can lead to diabetes mellitus or cancer.

4-Methylumbelliferyl-β-D-xylopyranoside is a fluorescent probe that has been used to visualize the enzyme activities of glycosidases, such as endoglycosidase H and α-amylase. It is also used as a tool in structural analysis of oligosaccharides. 4-Methylumbelliferyl-β-D-xylopyranoside has been shown to inhibit collagenase activity in mice, which may be due to its ability to inhibit protein synthesis and cell division.

Fluorogenic substrate for β-glucosidase

Beta-alanine 7-amido-4-methylcoumarin, trifluoroacetate salt is a high purity, ligand, and conjugate. It can be used in the food testing and environmental testing fields. This product has been shown to be a good substrate for enzyme activity and can also function as a fluorescent dye in chemiluminescence assays. Beta-alanine 7-amido-4-methylcoumarin, trifluoroacetate salt is soluble in water and has a CAS number of 201847-54-3.

2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid, sodium salt is a fluorogenic substrate for alpha-galactosidase. It is used in the analysis of cultured media and as a conjugate for enzyme substrates. This product has been shown to be useful in food testing, diagnostics, and environmental testing. 2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid, sodium salt has been used as a chromogenic substrate for the detection of alpha-galactosidase activity. This product is also used as a bioluminescent substrate for the detection of bioluminescence.

4-Methylumbelliferyl 3-O-(α-L-fucopyranosyl)-β-D-galactopyranoside is an organic compound that is used as a radioactive marker for DNA. It can be synthesized in the laboratory and its use allows for the tracking of DNA molecules. The production of this marker is achieved by recombinant expression of the gene encoding the enzyme β-D-galactosidase, which hydrolyzes 4-methylumbelliferyl β-D-galactopyranoside to release 4-methylumbelliferone and α-L-fucopyranose. The enzyme uses UDP glucose as a cofactor to catalyse the reaction. This product has been isolated from Corynebacterium glutamicum, with recombinant expression vectors used to produce it in host cells such as Escherichia coli or Corynebacterium glutamicum. It has been found that