Sustratos fluorogénicos

4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate potassium salt is a fluorogenic substrate for beta-N-acetylglucosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate potassium saltas a substrate for measuring the beta-N-acetylglucosaminidase activity is used for diagnosis of Mucopolysaccharidosis IIIB (MPS IIIB) (a rare lysosomal storage disorder) and Sanfilippo B syndrome screening.

N-alpha-CBZ-L-Arginine 7-amido-4-methylcoumarin hydrochloride is a Ffluorogenic substrate used in protease activity assays.

4-Methylumbelliferyl alpha-D-mannopyranoside 6-sulfate sodium salt is a fluorogenic substrate for sulfatase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl alpha-D-mannopyranoside 6-sulfate sodium saltas a substrate for measuring the sulfatase activity is used for Mucopolysaccharidosis (MPS) screening and other lysosomal storage disorder diagnosis.

Methyl 4-methylumbelliferyl hexyl phosphonate is a lipase suicide inhibitor that is a fluorogenic substrate for lipases which upon enzymatic action, binds irreversibly to the lipase and inactivates it. The substrate can be used for the determination of active sites in lipases and the fluorescent signal can be detected at Ex 363 nm and Em 465 nm.

3-Carboxyumbelliferyl b-D-glucuronide serves as a highly sensitive fluorogenic substrate for detecting b-glucuronidase activity. Upon enzymatic cleavage, it generates a fluorescent product enabling easy and accurate monitoring of enzyme activity in various applications, such as diagnostics, drug discovery, and research.

5-(Pentafluorobenzoylamino)fluoroscein di-b-D-galactopyranoside, commonly referred as PFB-FDG or PFB-FDGal, is a cell-permeable enzyme substrate for β-galactosidase used in live cell imaging assays. After cleavage, 5-(pentafluorobenzoylamino)fluorescein (PFB-F) is released, generating a green fluorescence (Em ~520 nm)) of a higher intensity than fluorescein. Its main use is for bacterial detection (e.g. E. coli LacZ assays), but also for flow cytometry analytical assays.

4-Methylumbelliferyl 2-O-(2,3,4-tri-O-benzoyl-a-L-fucopyranosyl)-b-D-galactopyranoside is a fluorescent substrate for β–galactosidase. It is used to detect the enzyme in biological samples and as a diagnostic agent. This substrate can be used to develop chemiluminescent, chromogenic, and fluorogenic substrates for use in diagnostics. 4MUF 2OBG can also be conjugated to various ligands for use in bioluminescence assays or as a marker in immunoassays.