Indicadores

Benzo[k]fluoranthene is a polycyclic aromatic hydrocarbon that is used as an optical sensor for transcriptional regulation. It has been shown to activate the transcription factor NF-κB, which induces expression of inflammatory genes in cells. Benzo[k]fluoranthene can be used to measure changes in gene expression and protein levels. The antibody response to benzo[k]fluoranthene was studied using a kinetic data analysis method. Benzo[k]fluoranthene has also been shown to induce genotoxic effects in liver cells and human hepatoma cell line HepG2. This compound also causes light emission when it is excited with light, which can be measured with synchronous fluorescence.

Produces a fluorogenic signal in the presence of peroxidase

Weakly fluorescent blue dye; indicator of mitochondrial activity. The reduction of resazurin has been ascribed to a putative reductase or diaphorase-type enzymes from the mitochondria and cytosol but also it might play a role as an intermediate electron acceptor in the electron transport chain between the final reduction of oxygen and cytochrome oxidase.

1,9-Dimethyl-methylene blue zinc chloride double salt is a fluorescent dye that has been used in the study of hyaluronic acid and mesenchymal stem cells. The compound absorbs light at a wavelength of 580 nm, which is the same as the absorption wavelength for hyaluronic acid and mesenchymal stem cells. 1,9-Dimethyl-methylene blue zinc chloride double salt can be used to measure the amount of these compounds in tissues. This dye also shows sensitivity to artifacts such as hemolysis and lipemia, making it useful for research purposes.