Enzimas en Proteínas Recombinantes

<p>Butyrylcholinesterase is an enzyme made in the liver and found mainly in blood plasma. Butyrylcholinesterase (EC 3.1.1.8), also known as BChE or BuChE, is a nonspecific cholinesterase enzyme that hydrolyses choline-based esters. One unit of Butyrylcholinesterase will hydrolyze 1.0 μmole of butyrylcholine to choline and butyrate per minute at pH 8.0 and 37 °C.</p>

<p>A proteolytic enzyme synthesized by the HIV cell as part of the GagPol polyprotein</p>

<p>Superoxide dismutase is an enzyme that catalyzes the conversion of harmful superoxide into hydrogen peroxide and oxygen.</p>

<p>DNase I (Deoxyribonuclease I, EC 3.1.21.1) is an endonuclease that cleaves DNA, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3'. On average it produces tetranucleotides. One unit of the DNase I will increase the absorbance of 260nm light at a rate of 0.001/minute in 1 ml reaction volume at 25°C.</p>

<p>Thioredoxin reductase (TR, TrxR) (EC 1.8.1.9) is an enzyme that reduce thioredoxin using NADPH as a co-factor, and also contains FAD. One unit of thioredoxin reductase will raise increase light absorbance by 1.0 per minute at 412nm in the presence of thioredoxin and Ellman's reagent at pH 7.0 and 25 °C.</p>

<p>Sulfatase from Helix pomatia is a highly potent enzyme that is capable of hydrolyzing sulfated compounds and sulfate esters. It has been widely used in various applications such as glucosinolate analysis, genistein extraction preparation, and regiospecificity studies. With a concentration of ≥10,000 units per gram of solid, this sulfatase offers exceptional enzymatic activity for sulfatase assays. It effectively catalyzes the hydrolysis of sulfated substrates, including p-nitrocatechol sulfate, naphthyl sulfate and phenyl sulfates.The enzyme can be incubated with the desired sample to facilitate the release of sulfate groups from sulfated compounds. Sulfatase from Helix pomatia is a valuable tool for researchers and scientists working in diverse fields requiring efficient and reliable enzymatic hydrolysis capabilities. Additionally the enzyme has been found to have industrial applications, such as in the bioconversion of industrial chemicals, where it can be used as a catalyst.</p>

<p>Triose-phosphate isomerase (TPI, TIM; EC 5.3.1.1) is an enzyme that catalyzes the reversible isomerisation of dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate:  DHAP ⇌ GADP  The reaction mechanism involves the formation of an enediol intermediate. One unit of Triose-phosphate isomerase will convert 1.0 μmole glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 and 25 °C.</p>

<p>Cholesterol oxidase (EC 1.1.3.6) is an enzyme that catalyzes the following reaction: cholesterol + O2 ⇌ cholest-4-en-3-one + H2O2One unit of cholesterol oxidase will convert 1.0 μmole of cholesterol into cholest-4-en-3-one per min at pH 7.5 and 25 °C. You can remove the build-up of hydrogen peroxide using catalase.</p>

<p>Glycerokinase (glycerol kinase, GP, ATP-glycerol 3-phosphotransferase; EC 2.7.1.30) is an enzyme that catalyzes the following reaction:  ATP + glycerol ⇌ ADP + glycerol 3-phosphate  One unit of Glycerokinase will convert 1.0 μmole of glycerol and ATP to glycerol 3-phosphate and ADP per min at pH 9.8 and 25 °C.</p>

<p>Oxalate Oxidase, freeze-dried, is an enzymatic preparation that serves as a catalyst in biochemical reactions. This enzyme is derived from wheat, a common plant source, ensuring a naturally occurring origin. Its primary mode of action is the oxidation of oxalate into carbon dioxide and hydrogen peroxide. This biochemical activity is significant in various scientific applications, specifically in the breakdown of oxalate, which plays a crucial role in metabolic and environmental processes.</p>

<p>Glycerol-3-phosphate oxidase (EC 1.1.3.21) is an enzyme that catalyzes the following reaction:  glycerol-3-phosphate + O2 ⇌ dihydroxyacetone phosphate + H2O2  One unit of Glycerol-3-phosphate oxidase will generate 1.0 μmole H2O2 per min at 37°C, under the presence of O2 and the optimal pH. If required, you can remove the build-up of hydrogen peroxide using catalase.</p>

<p>Phosphodiesterase II from bovine spleen is an enzyme derived from the spleen of cattle, which serves as a crucial biological catalyst for the hydrolysis of phosphodiester bonds in nucleotide sequences. This enzyme's mode of action involves cleaving the phosphodiester linkages within nucleic acids, facilitating the breakdown of these macromolecules into smaller nucleotide units.</p>

<p>Sarcosine oxidase (Monomeric sarcosine oxidase, MSOX, EC 1.5.3.1) is an enzyme that catalyzes the oxidative demethylation of sarcosine to yield glycine, H2O2 and formaldehyde in the following reaction:  CH3-NH2+-CH2-COO- + H2O + O2 → NH3+-CH2-COO- + H2O2 + CH2O or sarcosine + water + oxygen → glycine + hydrogen peroxyde + formaldehydeOne unit of Sarcosine oxidase will form 1.0 micromole of formaldehyde from sarcosine per minute at pH 8.3 and 37 °C. You can remove the build-up of hydrogen peroxide using catalase.</p>

<p>Poly(ethylene terephthalate) hydrolase is an enzyme involved in the breakdown of Polyethylene terephthalate which is present in many plastics Polyethylene terephthalate hydrolytic enzymes may be useful in biotechnology, for use in waste treatment, biocatalysis and biorecycling</p>

<p>Expression system: E. coli&lt;br&gt;Length: 1-169, Partial&lt;br&gt;Activity: BLI</p>

<p>Expression system: HEK297 Cells&lt;br&gt;Length: 21-472, Full Length of Mature Protein&lt;br&gt;Activity: Not Tested</p>

<p>Carboxypeptidase B2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.</p>

<p>ACE2/ACEH Protein, Human, Recombinant (hFc), Biotinylated is expressed in HEK293 mammalian cells with hFc tag.</p>

<p>Protein kinase C iota type, also known as Atypical protein kinase C-lambda/iota, aPKC-lambda/iota and PRKCI, is a cytoplasm, membrane and nucleus protein which</p>

<p>VEGFR3/FLT4 Protein, Human, Recombinant (His &amp; Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag.</p>