Enzimas

<p>BioLit QuickRxn EcoRI is expressed and purified from E.coli that carries the recombinant EcoRI gene. The molecular weight is 32.0 kDa. In molecular biology it is used as a restriction enzyme. It creates sticky ends with 5' end overhangs. The nucleic acid sequence where the enzyme cuts is GAATTC, which, as the complementary sequence is CTTAAG, has rotational symmetry. The reaction is conducted for 5 -15 minutes at 37°C ( Quick digestion with high fidelity) and heat-inactivated at 65°C for 20 minutes. Not sensitive to dam, dcm but Inhibition by CpG methylation. It is accompanied with the specific QuickRxn Buffer to enable 100 % enzyme activity.Concentration : 20U/mcl </p>

<p>Isoschizomers: FauNDI<br> Source: Neisseria denitrificans<br> 5'-CA↓TA TG-3'<br> 3'-GT AT↑AC-5 </p>

<p>PstI is an endonuclease enzyme isolated from strains of Providencia stuartii, recombinant, E.coli and is part of the restriction modification system.<br> In molecular biology it is used as a restriction enzyme. It creates sticky ends with 3' end overhangs. The nucleic acid sequence where the enzyme cuts is CTGCAG, which, as the complementary sequence is GACGTC, has rotational symmetry. </p>

Isoschizomers: AhlI, BcuI Sphaerotilus species A↓CTAG T-3' 3'-T GATC↑A-5'

MspCI is an endonuclease enzyme isolated from strains of Micrococcus species and is part of the restriction modification system. It is an isoschizomer of AflII. In molecular biology it is used as a restriction enzyme. It creates sticky ends with a 3' overhang. The nucleic acid sequence where the enzyme cuts is CTTAAG, which, as the complementary sequence is GAATTC, has rotational symmetry.

<p>Isoschizomers: HindII<br> Source: Haemophilus influenzae Rc<br> 5'-GTY↓RAC-3'<br> 3'-CAR↑YTG-5 </p>

<p>SacI is an Isoschizomers of SstI,<br> Neoschizomers: Ecl136II, EcoICRI<br> Source: Streptomyces Stanford<br> 5'-G AGCT↓C-3'<br> 3'-C↑TCGA G-5' </p>