Mca-(Ala⁷,Lys(Dnp)⁹)-Bradykinin

CAS: 323577-36-2

Ref. 01-4029459

Información del producto

• Nombre: Mca-(Ala⁷,Lys(Dnp)⁹)-Bradykinin
• Sinónimos: L-Lysine,N2-[(7-methoxy-2-oxo-2H-1-benzopyran-4-yl)acetyl]-L-arginyl-L-prolyl-L-prolylglycyl-L-phenylalanyl-L-seryl-L-alanyl-L-phenylalanyl-N6-(2,4-dinitrophenyl)-(9CI), 61: PN: WO03065997 SEQID: 91 unclaimed protein, Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-Oh
• CAS: 323577-36-2

Descripción

Substrate V, Mca-RPPGFSAFK(Dnp), is a very sensitive internally quenched fluorescent substrate for endothelin-converting enzyme-1 (ECE-1) a membrane-bound zinc metallopeptidase that is related to neprilysin in amino acid sequence. The kcat/Km value for the hydrolysis by ECE-1 was 1.9 x 107 M-1s-1 and 1.7 x 106 M-1s-1 for the hydrolysis by neprilysin. This FRET substrate was hydrolyzed rapidly by stromelysin 1 (MMP-3) with kcat/Km= 218000 M-1s-1 and very slowly by gelatinase B (MMP-9) (kcat/Km= 10100 M-1s-1). There was no hydrolysis by MMP-1 and MMP-2. Mca-RPPGFSAFK(Dnp) has also found use as substrate for insulin-degrading enzyme (IDE) and ACE2.

• Marca: Bachem

Propiedades químicas

• Peso molecular: 1388.46
• Fórmula: C₆₆H₈₁N₁₅O₁₉
• Pureza: 98.5%
• Color/Forma: Yellow Lyophilisate