Insulysin FRET substrate [Mca]/[Dnp]

Ref. 3D-CRB1100406

Información del producto

• Nombre: Insulysin FRET substrate [Mca]/[Dnp]
• Sinónimos: Mca-GGFLRKHGQ-Dnp[Mca]-GGFLRKHGQ-[Dnp][Mca]-Gly-Gly-Phe-Leu-Arg-Lys-His-Gly-Gln-[Dnp], Where Mca is 7-methoxycoumarin-4-acetyl and Dnp is ethylene diamine-N-2,4-dinitrophenyl

Descripción

The Insulysin FRET substrate [Mca]/[Dnp] is a synthetic insulysin peptide substrate that contains an N-terminal fluorescent 7-methoxycoumarin (Mca) group and a C-terminal 2, 4-dinitrophenyl (Dnp) quencher. This FRET peptide exhibits internal fluorescence quenching when intact, however hydrolysis of the peptide between the donor/acceptor pair generates fluorescence, enabling the quantitative measure of enzymatic activity.Insulysin, also known as insulin-degrading enzyme (IDE), is a highly conserved zinc metallopeptidase that contains an inverted zincin motif (HXXEH), characteristic of the inverzincin family of proteases. Insulysin functions as a dimer and exhibits allosteric kinetics, whereby small substrate peptides and polyphosphates such as ATP can act as enzyme activators. The endopeptidase cleaves a wide variety of substrates that are diverse in length, structure and sequence and exhibits a preference for basic or bulky hydrophobic residues.Insulysin is ubiquitously expressed and is responsible for insulin catabolism and the degradation of many bioactive peptide substrates including glucagon, TGF-alpha, β-endorphin, dynorphins and atrial natriuretic peptide. Insulysin also selectively degrades amylin and β-amyloid amyloidogenic peptides at multiple sites.Fluorescence Resonance Energy Transfer (FRET) peptides are convenient tools for the study of peptidase specificity and enzymatic activity.

• Marca: Biosynth

Propiedades químicas

• Peso molecular: 1,422.6 g/mol