3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate

CAS: 207738-08-7

Ref. 3D-FT170921

Información del producto

• Nombre: 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate
• Sinónimos: TMB dihydrochloride

Descripción

HRP and TMB - it takes two to tango! The enzyme horseradish peroxidase (HRP) has been isolated from the roots of the plant in commercially useful quantities since the nineteen-sixties. Today, HRP-coupled antibodies have become indispensable in immunoassays. The most popular chromogenic substrate for HRP is Tetramethylbenzidine (TMB), in particular in enzyme-linked Immunosorbent assays (ELISA). HRP converts TMB into a blue color that can be read spectrophotometrically at 650 nm. The HRP reaction can be stopped by adding sulfuric or phosphoric acid. This yields a yellow color that can be read at 450 nm.

• Marca: Biosynth

Propiedades químicas

• Peso molecular: 313.3 g/mol
• Fórmula: C₁₆H₂₀N₂·2HCl·xH₂O
• Pureza: Sodium Hydroxide Titration -
• Color/Forma: Powder