EasyStep Mouse 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit

Ref. EK-ELK052ES

Información del producto

• Nombre: EasyStep Mouse 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
• Sinónimos: 25 Hydroxy Vitamin D

Descripción

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 25-OH-D antigen, and the Mouse 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marca: ELK Biotechnology