Human SLC17A5(Sialin) ELISA Kit

Ref. EK-ELK0725

Información del producto

• Nombre: Human SLC17A5(Sialin) ELISA Kit
• Sinónimos: H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter (AST); Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular H(+)/Aspartate-glutamate cotransporter

Descripción

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SLC17A5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SLC17A5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SLC17A5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SLC17A5 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marca: ELK Biotechnology