Human APOF(Apolipoprotein F) ELISA Kit
Ref. EK-ELK2250
48T | 401,00 € | ||
96T | 576,00 € |
Información del producto
- Apo-F; LTIP; Lipid transfer inhibitor protein
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOF in the samples is then determined by comparing the OD of the samples to the standard curve.
Propiedades químicas
Consulta técnica sobre: EK-ELK2250 Human APOF(Apolipoprotein F) ELISA Kit
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