Human EAAT1(Excitatory Amino Acid Transporter 1) ELISA Kit

Ref. EK-ELK3929

Información del producto

• Nombre: Human EAAT1(Excitatory Amino Acid Transporter 1) ELISA Kit
• Sinónimos: SLC1A3; EA6; GLAST1; Sodium-dependent glutamate/aspartate transporter 1; Solute Carrier 1 Member 3,Glial High Affinity Glutamate Transporter; GLutamate Aspartate Transporter

Descripción

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EAAT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EAAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EAAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EAAT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marca: ELK Biotechnology