Human KIF5A(Kinesin Family, Member 5A) ELISA Kit

Ref. EK-ELK3930

Información del producto

• Nombre: Human KIF5A(Kinesin Family, Member 5A) ELISA Kit
• Sinónimos: NKHC; MY050; SPG10; Spastic Paraplegia 10(Autosomal Dominant); Kinesin heavy chain neuron-specific 1; Neuronal kinesin heavy chain

Descripción

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KIF5A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KIF5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KIF5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KIF5A in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marca: ELK Biotechnology