Rat AGT(Angiotensinogen) ELISA Kit
Ref. EK-ELK4678
48T | 402,00 € | ||
96T | 574,00 € |
Información del producto
- ANHU; SERPINA8; Angiotensin; Renin Substrate; Serpin Peptidase Inhibitor,Clade A,Member 8(Alpha-1 Antiproteinase,Antitrypsin)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AGT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AGT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AGT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AGT in the samples is then determined by comparing the OD of the samples to the standard curve.
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Consulta técnica sobre: EK-ELK4678 Rat AGT(Angiotensinogen) ELISA Kit
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