Mouse IgM(Immunoglobulin M) ELISA Kit
Ref. EK-ELK5383
48T | 402,00 € | ||
96T | 575,00 € |
Información del producto
- IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse IgM protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IgM in the samples is then determined by comparing the OD of the samples to the standard curve.
Propiedades químicas
Consulta técnica sobre: EK-ELK5383 Mouse IgM(Immunoglobulin M) ELISA Kit
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