Rat CFB(Complement Factor B) ELISA Kit
Ref. EK-ELK5823
48T | 402,00 € | ||
96T | 574,00 € |
Información del producto
- CF-B; BF; BFD; CFAB; GBG; H2-Bf; PBF2; B-Factor; Properdin; Properdin factor B; C3/C5 convertase; Glycine-rich beta glycoprotein
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CFB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CFB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CFB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CFB in the samples is then determined by comparing the OD of the samples to the standard curve.
Propiedades químicas
Consulta técnica sobre: EK-ELK5823 Rat CFB(Complement Factor B) ELISA Kit
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