Human DEXI(Dexamethasone Induced Protein) ELISA Kit
Ref. EK-ELK6999
48T | 401,00 € | ||
96T | 577,00 € |
Información del producto
- MYLE; Dexamethasone Induced Transcript
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DEXI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DEXI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DEXI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DEXI in the samples is then determined by comparing the OD of the samples to the standard curve.
Propiedades químicas
Consulta técnica sobre: EK-ELK6999 Human DEXI(Dexamethasone Induced Protein) ELISA Kit
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