Mouse TWIST(Twist Transcription Factor) ELISA Kit

Ref. EK-ELK7631

Información del producto

• Nombre: Mouse TWIST(Twist Transcription Factor) ELISA Kit
• Sinónimos: ACS3; BPES2; BPES3; SCS; Class A basic helix-loop-helix protein 38; Acrocephalosyndactyly 3; Saethre-Chotzen Syndrome; Blepharophimosis,Epicanthus Inversus And Ptosis 3

Descripción

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TWIST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TWIST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TWIST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TWIST in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marca: ELK Biotechnology